Thermodynamic Analysis of Nylon Nucleic Acids

Abstract

The stability and structure of nylon nucleic acid duplexes with complementary DNA and RNA strands was examined. Thermal denaturing studies of a series of oligonucleotides that contained nylon nucleic acids (1-5 amide linkages) revealed that the amide linkage significantly enhanced the binding affinity of nylon nucleic acids towards both complementary DNA (up to 26°C) increase in the thermal transition temperature (Tm) for five linkages) and RNA (around 15°C increase in Tm for five linkages) compared with nonamide linked precursor strands. For both DNA and RNA complements, increasing derivatization decreased the melting temperatures of uncoupled molecules relative to unmodified strands; by contrast, increasing lengths of coupled copolymer raised Tm from less to slightly greater than Tm of unmodified strands. Thermodynamic data extracted from melting curves and CD spectra of nylon nucleic acid duplexes were consistent with loss of stability due to incorporation of pendent groups on the 2'-position of ribose and recovery of stability upon linkage of the side chains.

Department(s)

Chemistry

Keywords and Phrases

Complementary DNA; Copolymer; Nucleic Acid; Nylon; Oligonucleotide; RNA; Complementary DNA; Complementary RNA; DNA; Oligodeoxyribonucleotide; Binding Affinity; Circular Dichroism; Conformational Transition; Controlled Study; DNA Binding; DNA Denaturation; DNA RNA Hybridization; DNA Sequence; Melting Point; Nucleic Acid Hybridization; Nucleic Acid Structure; Priority Journal; Thermodynamics; Transition Temperature; Chemistry; DNA Template; Kinetics; Nucleotide Sequence; Thermodynamics; Base Sequence; Kinetics; Nylons; Oligodeoxyribonucleotides

International Standard Serial Number (ISSN)

1439-4227

Document Type

Article - Journal

Document Version

Citation

File Type

text

Language(s)

English

Rights

© 2008 Wiley, All rights reserved.

Publication Date

01 Jul 2008

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