Transition Metal Ion Binding Studies of Carnosine and Histidine: Biologically Relevant Antioxidants

Abstract

Carnosine and histidine are biologically interesting antioxidants. In order to probe whether they exert their antioxidant effect through metal ion chelation, the Cu(II) ion chelating abilities of these compounds were measured by UV-vis spectroscopy. Both of these compounds showed 1:1 complexations with Cu(II) ions as shown by their Job's plot. The binding constants for histidine and carnosine, as determined by Benesi-Hildebrand method, at pH 7.84 ± 0.18, were found to be 71 and 1.1 M-1, respectively. The unexpectedly lower binding constant values of carnosine show the relatively minor role of the transition metal ion chelation in their antioxidant abilities.

Department(s)

Chemistry

Keywords and Phrases

Arsenic compounds; Chelation; Chlorine compounds; Copper alloys; Ions; Metal ions; Metals; Transition metal compounds; Transition metals; Ultraviolet spectroscopy; Antioxidant; Antioxidant effect; Benesi-Hildebrand; Binding constants; Carnosine; Cu (II); Histidine; Job's plot; Metal ion binding; Transition-metal ions; UV-VIS spectroscopy; Complexation; chelating agent; copper ion; binding affinity; chelation; complex formation; methodology; pH; priority journal; Binding Sites; Binding, Competitive; Hydrogen-Ion Concentration; Ions; Molecular Structure; Spectrophotometry, Ultraviolet; Surface Properties; Carnosine

International Standard Serial Number (ISSN)

0927-7765

Document Type

Article - Journal

Document Version

Citation

File Type

text

Language(s)

English

Rights

© 2008 Elsevier, All rights reserved.

Publication Date

01 Oct 2008

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