Uptake and Enantioselective Elimination of Chlordane Compounds by Common Carp (Cyprinus Carpio, L.)
An analytical method involving supercritical fluid extraction (SFE) followed by a two-dimensional gas chromatography (2D-GC) analysis was developed to determine the concentration (first GC) and enantiomeric ratio (second GC) of cis- and trans-chlordanes at the ppb (ng/g) level in fish tissue. The SFE method allowed concentration of the compounds of interest, and reduced the number of extraction and sample clean-up manipulations as compared to classical solvent extraction techniques. Four hundred common carp fingerling (Cyprinus carpio, L.) were exposed for three days to water containing 5 ppb (5 ng/g) technical grade chlordane containing about 1 ppb of chlordane isomers. The fish concentrated the pesticides more than 200 times (162 and 312 ng/g of cis- and trans-chlordane, respectively). However, the uptake is not enantioselective. The concentration of the principle constituents and their enantiomeric ratio was followed during a fifty days growth period in chlordane free water. The first order decay of concentration was observed with a half time of about 18 days for both the cis- and trans-chlordane isomers. However it was found that the enantiomeric ratio of the trans-chlordane was significantly altered during this short period of time, decreasing from ER = 1 to ER = 0.7, while no enantiomeric changes were observed for the cis-chlordane. It seems that the (-)-trans-chlordane is metabolized significantly faster (t1/2- = 15 days) in the river carp fish than the (+)-trans-enantiomer (t1/2+ = 20 days). © 2005 Elsevier Ltd. All rights reserved.
R. Seemamahannop et al., "Uptake and Enantioselective Elimination of Chlordane Compounds by Common Carp (Cyprinus Carpio, L.)," Chemosphere, Elsevier, Jan 2005.
The definitive version is available at http://dx.doi.org/10.1016/j.chemosphere.2004.11.101
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© 2005 Elsevier, All rights reserved.