Uptake and Enantioselective Elimination of Chlordane Compounds by Common Carp (Cyprinus Carpio, L.)

Abstract

An analytical method involving supercritical fluid extraction (SFE) followed by a two-dimensional gas chromatography (2D-GC) analysis was developed to determine the concentration (first GC) and enantiomeric ratio (second GC) of cis- and trans-chlordanes at the ppb (ng/g) level in fish tissue. The SFE method allowed concentration of the compounds of interest, and reduced the number of extraction and sample clean-up manipulations as compared to classical solvent extraction techniques. Four hundred common carp fingerling (Cyprinus carpio, L.) were exposed for three days to water containing 5 ppb (5 ng/g) technical grade chlordane containing about 1 ppb of chlordane isomers. The fish concentrated the pesticides more than 200 times (162 and 312 ng/g of cis- and trans-chlordane, respectively). However, the uptake is not enantioselective. The concentration of the principle constituents and their enantiomeric ratio was followed during a fifty days growth period in chlordane free water. The first order decay of concentration was observed with a half time of about 18 days for both the cis- and trans-chlordane isomers. However it was found that the enantiomeric ratio of the trans-chlordane was significantly altered during this short period of time, decreasing from ER = 1 to ER = 0.7, while no enantiomeric changes were observed for the cis-chlordane. It seems that the (-)-trans-chlordane is metabolized significantly faster (t1/2- = 15 days) in the river carp fish than the (+)-trans-enantiomer (t1/2+ = 20 days). © 2005 Elsevier Ltd. All rights reserved.

Department(s)

Chemistry

International Standard Serial Number (ISSN)

0045-6535

Document Type

Article - Journal

Document Version

Citation

File Type

text

Language(s)

English

Rights

© 2005 Elsevier, All rights reserved.

Publication Date

01 Jan 2005

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