Correlations between Molecular Numbers and Molecular Masses in an Evanescent Field and their Applications in Probing Molecular Interactions

Author

Hongwei Gai
Qi Wang
Yinfa Ma, Missouri University of Science and TechnologyFollow
Bingcheng Lin

Abstract

Complex numbers: The number of molecules detected in the evanescent field during single-molecule fluorescence imaging decreases with increasing molecular weight in free solution (see graphic; FITC = fluorescein isothiocyanate). This technique could be useful for screening undefined molecular interactions at extremely low concentrations without separation.

Recommended Citation

H. Gai et al., "Correlations between Molecular Numbers and Molecular Masses in an Evanescent Field and their Applications in Probing Molecular Interactions," Angewandte Chemie International Edition, vol. 44, no. 32, pp. 5107 - 5110, Wiley-VCH Verlag Berlin, Aug 2005.

The definitive version is available at https://doi.org/10.1002/ange.200500461

Department(s)

Chemistry

Keywords and Phrases

Evanescent Field; Fluorescence; Molecule Counting; Oligonucleotides; Single-molecule Studies

International Standard Serial Number (ISSN)

1433-7851

Document Type

Article - Journal

Document Version

Citation

File Type

text

Language(s)

English

Rights

© 2005 Wiley-VCH Verlag Berlin, All rights reserved.

Publication Date

01 Aug 2005