Click Hybridization of Immune Cells and Polyamidoamine Dendrimers

Abstract

Immobilizing highly branched polyamidoamine (PAMAM) dendrimers to the cell surface represents an innovative method of enhancing cell surface loading capacity to deliver therapeutic and imaging agents. In this work, hybridized immune cells, that is, macrophage RAW264.7 (RAW), with PAMAM dendrimer G4.0 (DEN) on the basis of bioorthogonal chemistry are clicked. Efficient and selective cell surface immobilization of dendrimers is confirmed by confocal microscopy. Viability and motility of RAW-DEN hybrids remain the same as untreated RAW cells according to WST-1 assay and wound closure assay. Furthermore, Western blot analysis reveals that there are no significant alterations in the expression levels of signaling molecules AKT, p38, and NFκB (p65) and their corresponding activated (phosphorylated) forms in RAW cells treated with azido sugar and dendrimer, indicating that the hybridization process neither induced cell stress response nor altered normal signaling pathways. Taken together, this work shows the feasibility of applying bioorthogonal chemistry to create cell-nanoparticle hybrids and demonstrates the noninvasiveness of this cell surface engineering approach.

Department(s)

Chemical and Biochemical Engineering

Keywords and Phrases

Cell engineering; Cell immobilization; Cell membranes; Cytology; Dendrimers; Electrophoresis; Functional polymers, Bioorthogonal chemistries; Cell surface engineering; Hybridization process; Macrophage RAW264.7; Polyamidoamine dendrimers; Signaling molecules; Signaling pathways; Western-blot analysis, Cell signaling, benzyl derivative; dendrimer; dibenzocyclooctyne; immobilizing highly branched polyamidoamine dendrimer; nanoparticle; polyamidoamine; protein kinase B; synaptophysin; transcription factor RelA; unclassified drug; dendrimer; polyamidoamine; polyamine, animal cell; Article; cell assay; cell motility; cell surface; cell viability; click chemistry; click hybridization; confocal microscopy; controlled study; hybridization; immobilized cell; immunocompetent cell; internalization; intracellular signaling; macrophage; mouse; non invasive procedure; nonhuman; protein expression; protein phosphorylation; structure analysis; Western blotting; animal; cell motion; cell survival; chemistry; cytology; drug effects; materials testing; metabolism; procedures; signal transduction, Animals; Cell Movement; Cell Survival; Click Chemistry; Dendrimers; Macrophages; Materials Testing; Mice; Nanoparticles; Polyamines; Signal Transduction

International Standard Serial Number (ISSN)

2192-2640; 2192-2659

Document Type

Article - Journal

Document Version

Citation

File Type

text

Language(s)

English

Rights

© 2014 Wiley-VCH Verlag, All rights reserved.

Publication Date

01 Sep 2014

Link to Full Text

Share

 
COinS