Effect of Glycosylation on the Partition Behavior of a Human Antibody in Aqueous Two-Phase Systems
Abstract
Human proteins are expressed in some hosts wrongly glycosylated or non-glycosylated. Although it is accepted that glycosylation contributes to the stability of the protein in solution, the effect of glycosylation on the stability of human antibodies is not fully understood. In this work, we present solubility studies of two human antibodies that have the same primary structure but different glycosylation pattern. The studies were done by monitoring the partitioning behavior of both proteins in a series of aqueous two-phase systems at and away the isoelectric point of the proteins and at different temperatures. Our studies show that in the absence of direct electrostatic forces, the partitioning behavior of the antibodies depends on the presence or absence of the polysaccharide chains. Overall, the non-glycosylated protein is less soluble than the glycosylated one. The potential of aqueous two-phase systems for the separation of the glycosylated and non-glycosylated proteins was also explored. A simple series of extractions seems to be enough to separate the glycosylated variety from the non-glycosylated one at high purity but low yields.
Recommended Citation
J. Lee and D. Forciniti, "Effect of Glycosylation on the Partition Behavior of a Human Antibody in Aqueous Two-Phase Systems," Proceedings of the AIChE Annual Meeting (2011, Minneapolis, MN), American Institute of Chemical Engineers (AIChE), Oct 2011.
Meeting Name
AIChE Annual Meeting (2011: Oct. 16-21, Minneapolis, MN)
Department(s)
Chemical and Biochemical Engineering
Keywords and Phrases
Aqueous Two Phase System; Glycosylated; High Purity; Human Antibodies; Human Proteins; Iso-Electric Points; Low-Yield; Partition Behaviors; Polysaccharide Chain; Primary Structures; Solubility Studies; Behavioral Research; Esterification; Glycosylation; Separation; Antibodies
International Standard Book Number (ISBN)
978-0-8169-1070-0
Document Type
Article - Conference proceedings
Document Version
Citation
File Type
text
Language(s)
English
Rights
© 2011 American Institute of Chemical Engineers (AIChE), All rights reserved.
Publication Date
01 Oct 2011
