Bioresponsive Polymer Coated Drug Nanorods for Breast Cancer Treatment

Abstract

Ineffective drug release at the target site is among the top challenges for cancer treatment. This reflects the facts that interaction with the physiological condition can denature active ingredients of drugs, and low delivery to the disease microenvironment leads to poor therapeutic outcomes. We hypothesize that depositing a thin layer of bioresponsive polymer on the surface of drug nanoparticles would not only protect drugs from degradation but also allow the release of drugs at the target site. Here, we report a one-step process to prepare bioresponsive polymer coated drug nanorods (NRs) from liquid precursors using the solvent diffusion method. A thin layer (10.3 ± 1.4 nm) of poly(ε-caprolactone) (PCL) polymer coating was deposited on the surface of camptothecin (CPT) anti-cancer drug NRs. The mean size of PCL-coated CPT NRs was 500.9 ± 91.3 nm length x 122.7 ± 10.1 nm width. The PCL polymer coating was biodegradable at acidic pH 6 as determined by Fourier transform infrared spectroscopy. CPT drugs were released up to 51.5% when PCL coating dissolved into non-toxic carboxyl and hydroxyl groups. Trastuzumab (TTZ), a humanized IgG monoclonal antibody, was conjugated to the NR surface for breast cancer cell targeting. Combination treatments using CPT and TTZ decreased the HER-2 positive BT-474 breast cancer cell growth by 66.9 ± 5.3% in vitro. These results suggest effective combination treatments of breast cancer cells using bioresponsive polymer coated drug delivery.

Department(s)

Chemical and Biochemical Engineering

Second Department

Biological Sciences

Keywords and Phrases

Antibodies; Biodegradable Polymers; Body Fluids; Cells; Coatings; Deposition; Diseases; Drug Delivery; Fourier Transform Infrared Spectroscopy; Monoclonal Antibodies; Nanorods; Polycaprolactone; Polymers; Breast Cancer Treatment; Camptothecin (CPT); Cancer Therapy; Combination Treatments; Physiological Condition; Solvent Diffusion Methods; Therapeutic Outcomes; Trastuzumab; Plastic Coatings

International Standard Serial Number (ISSN)

0957-4484; 1361-6528

Document Type

Article - Journal

Document Version

Citation

File Type

text

Language(s)

English

Rights

© 2017 Institute of Physics - IOP Publishing, All rights reserved.

Publication Date

01 Jan 2017

PubMed ID

27977417

Link to Full Text

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