From Solvent-free Microspheres To Bioactive Gradient Scaffolds
Abstract
A solvent-free microsphere sintering technique was developed to fabricate scaffolds with pore size gradient for tissue engineering applications. Poly(D,L-Lactide) microspheres were fabricated through an emulsification method where TiO2 nanoparticles were employed both as particulate emulsifier in the preparation procedure and as surface modification agent to improve bioactivity of the scaffolds. A fine-tunable pore size gradient was achieved with a pore volume of 30 ± 2.6%. SEM, EDX, XRD and FTIR analyses all confirmed the formation of bone-like apatite at the 14th day of immersion in Simulated Body Fluid (SBF) implying the ability of our scaffolds to bond to living bone tissue. In vitro examination of the scaffolds showed progressive activity of the osteoblasts on the scaffold with evidence of increase in its mineral content. The bioactive scaffold developed in this study has the potential to be used as a suitable biomaterial for bone tissue engineering and hard tissue regeneration.
Recommended Citation
M. Rasoulianboroujeni and M. Yazdimamaghani and P. Khoshkenar and V. R. Pothineni and K. M. Kim and T. A. Murray and J. Rajadas and D. K. Mills and D. Vashaee and K. Moharamzadeh and L. Tayebi, "From Solvent-free Microspheres To Bioactive Gradient Scaffolds," Nanomedicine Nanotechnology Biology and Medicine, vol. 13, no. 3, pp. 1157 - 1169, Elsevier, Apr 2017.
The definitive version is available at https://doi.org/10.1016/j.nano.2016.10.008
Department(s)
Chemical and Biochemical Engineering
Keywords and Phrases
Bone-like apatite; Microsphere sintering; Mineralization; Pore size gradient; Solvent-free method; Tissue engineering scaffold
International Standard Serial Number (ISSN)
1549-9642; 1549-9634
Document Type
Article - Journal
Document Version
Citation
File Type
text
Language(s)
English
Rights
© 2025 Elsevier, All rights reserved.
Publication Date
01 Apr 2017
PubMed ID
27793788
Comments
National Science Foundation, Grant 1363485