Abstract
Slight alterations in nanoparticles' surface properties can significantly influence the corona composition which may alter their interaction with the biological milieu. Size and porosity of silica nanoparticles (SNPs) are known to be predominant factors influencing their dose-dependent toxicity. Little is known however about the extent and type of protein adsorption on SNPs as a function of physicochemical properties and the role this might play on mechanisms of cellular uptake and toxicity. In this work we investigated the influence of size and porosity of SNPs on protein adsorption, cellular uptake, and toxicity in RAW 264.7 macrophages. Toxicity of the SNPs was found to be concentration dependent, and the formation of the protein corona mitigated toxicity for all particles. Detailed analysis of the amount of proteins recovered from each nanoparticle revealed similarities in the protein adsorption profile as a function of size and porosity. The mechanism of uptake was highly dependent on size rather than porosity or the adsorbed proteins.
Recommended Citation
J. Saikia et al., "Differential Protein Adsorption And Cellular Uptake Of Silica Nanoparticles Based On Size And Porosity," ACS Applied Materials and Interfaces, vol. 8, no. 50, pp. 34820 - 34832, American Chemical Society, Dec 2016.
The definitive version is available at https://doi.org/10.1021/acsami.6b09950
Department(s)
Chemical and Biochemical Engineering
Keywords and Phrases
cellular uptake; cytotoxicity; protein adsorption; protein corona; silica nanoparticles
International Standard Serial Number (ISSN)
1944-8252; 1944-8244
Document Type
Article - Journal
Document Version
Citation
File Type
text
Language(s)
English
Rights
© 2025 American Chemical Society, All rights reserved.
Publication Date
21 Dec 2016
Comments
National Institutes of Health, Grant R01ES024681