Global Gene Expression Analysis Of Macrophage Response Induced By Nonporous And Porous Silica Nanoparticles

Abstract

Little is known about the global gene expression profile of macrophages in response to changes in size and porosity of silica nanoparticles (SNPs). Spherical nonporous SNPs of two different diameters, and mesoporous spherical SNPs with comparable size were characterized. Reactive oxygen species, mitochondrial membrane potential, lysosome degradation capacity, and lysosome pH were measured to evaluate the influence of nonporous and mesoporous SNPs on mitochondrial and lysosomal function. RNA-sequencing was utilized to generate transcriptional profiles of RAW264.7 macrophages exposed to non-toxic SNP doses. DESeq2, limma, and BinReg2 software were used to analyze the data based on both unsupervised and supervised strategies to identify genes with greatest differences among NP treatments. Utilizing GATHER and DAVID software, possible induced pathways were studied. We found that mesoporous silica nanoparticles are capable of altering gene expression in macrophages at doses that do not elicit acute cytotoxicity, while gene transcription was minimally affected by nonporous SNPs.

Department(s)

Chemical and Biochemical Engineering

Comments

National Science Foundation, Grant P30 CA042014

Keywords and Phrases

Gene expression; Gene ontology; Lysosome pathway; Nanotoxicity; Reactive oxygen species; Silica nanoparticles

International Standard Serial Number (ISSN)

1549-9642; 1549-9634

Document Type

Article - Journal

Document Version

Citation

File Type

text

Language(s)

English

Rights

© 2025 Elsevier, All rights reserved.

Publication Date

01 Feb 2018

PubMed ID

29203145

Link to Full Text

Share

 
COinS