Abstract
17β-Estradiol (E2) confers neuroprotection in preclinical models of spinal cord injury when administered systemically. The goal of this study was to apply E2 locally to the injured spinal cord for a sustained duration using poly(pro-E2) film biomaterials. Following contusive spinal cord injury in adult male mice, poly(pro-E2) films were implanted subdurally and neuroprotection was assessed using immunohistochemistry 7 days after injury and implantation. In these studies, poly(pro-E2) films modestly improved neuroprotection without affecting the inflammatory response when compared to the injured controls. To increase the E2 dose released, bolus-releasing poly(pro-E2) films were fabricated by incorporating unbound E2 into the poly(pro-E2) films. However, compared to the injured controls, bolus-releasing poly(pro-E2) films did not significantly enhance neuroprotection or limit inflammation at either 7- or 21-days post-injury. Future work will focus on developing poly(pro-E2) biomaterials capable of more precisely releasing therapeutic doses of E2.
Recommended Citation
M. K. Gottipati et al., "Acute Dose-Dependent Neuroprotective Effects of Poly(pro-17β-estradiol) in a Mouse Model of Spinal Contusion Injury," ACS Chemical Neuroscience, vol. 12, no. 6, pp. 959 - 965, American Chemical Society, Mar 2021.
The definitive version is available at https://doi.org/10.1021/acschemneuro.0c00798
Department(s)
Chemical and Biochemical Engineering
Keywords and Phrases
Estrogen; inflammation; neuroprotection; poly(prodrug); spinal cord injury
International Standard Serial Number (ISSN)
1948-7193
Document Type
Article - Journal
Document Version
Citation
File Type
text
Language(s)
English
Rights
© 2025 American Chemical Society, All rights reserved.
Publication Date
17 Mar 2021
PubMed ID
33635633
Comments
National Institutes of Health, Grant C32245GG