Abstract
We hypothesized that fiber diameter within an electro spun fiber scaffold would affect astrocyte elongation and maturation. To examine the relationship, astrocytes were seeded on 400nm and 800nm diameter electro spun PLLA fibers. Immunohistochemistry was used to monitor cell morphology, and GLT-1 expression over the first 96 hours after seeding. The results indicate that astrocytes extended along the primary axis of the 800nm fibers more rapidly than astrocytes seeded on fibers of half the size. While GLT-1 was expressed diffusely throughout the surface of the astrocytes, elongated cell processes exhibited dense regions of GLT-1. These results show that 800nm fibers allowed astrocytes to develop GLT-1 rich cellular processes more rapidly than 400nm fibers. These findings indicate that fiber diameter is an important parameter to consider when designing materials that regulate astrocyte physiology.
Recommended Citation
C. D. Johnson et al., "Initial Astrocyte Response to Electrospun Poly-l-lactic Acid Fibers of Varying Nanoscale Diameters," 2015 41st Annual Northeast Biomedical Engineering Conference Nebec 2015, article no. 7117048, Institute of Electrical and Electronics Engineers, Jun 2015.
The definitive version is available at https://doi.org/10.1109/NEBEC.2015.7117048
Department(s)
Chemical and Biochemical Engineering
Keywords and Phrases
Actin; Astrocyte; Electrospun fibers; GLT-1
International Standard Book Number (ISBN)
978-147998360-5
Document Type
Article - Conference proceedings
Document Version
Citation
File Type
text
Language(s)
English
Rights
© 2025 Institute of Electrical and Electronics Engineers, All rights reserved.
Publication Date
02 Jun 2015
