Abstract

We describe the patterning of neuronal cultures by a laser-based printing system. Embryonic chick cortical neurons were transferred into predetermined array positions with micron accuracy, enabling detailed study of neuronal migration, aggregation, and neurite outgrowth regulated by cell-cell interactions on a homogeneous surface. Images of cells were analyzed up to 14 days. Results demonstrated that increasing the spacing between neighboring groups of cells causes intra-group cells to preferentially migrate towards each other and aggregate towards the center of the printed 'spot'. In addition, increasing the printed 'spot' size encouraged migration towards neighboring groups of cells. © 2012 IEEE.

Department(s)

Chemical and Biochemical Engineering

International Standard Book Number (ISBN)

978-146731141-0

Document Type

Article - Conference proceedings

Document Version

Citation

File Type

text

Language(s)

English

Rights

© 2025 Institute of Electrical and Electronics Engineers, All rights reserved.

Publication Date

29 Jun 2012

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