Abstract

Purpose: Superparamagnetic iron oxide nanoparticles are currently approved for use as an adjunctive treatment to glioblastoma multiforme radiotherapy. Radio frequency stimulation of the nanoparticles generates localized hyperthermia, which sensitizes the tumor to the effects of radiotherapy. Clinical trials reported thus far are promising, with an increase in patient survival rate; however, what are left unaddressed are the implications of this technology on the surrounding healthy tissue. Methods and materials: Amino silane-coated iron oxide nanoparticles suspended in culture medium were applied to chick embryonic cortical neuron cultures. Cultures were heated to 37 °C or 45 °C by an induction coil system for 2 h. The latter regime emulates the therapeutic conditions of the adjunctive therapy. Cellular viability and neurite retraction was quantified 24 h after exposure to the hyperthermic events. Results: The hyperthermic load inflicted little damage to the neuron cultures, as determined by calcein-AM, propidium iodide, and Alamar Blue® assays. Fluorescence imaging was used to assess the extent of neurite retraction which was found to be negligible. Conclusions: Retention of chick, embryonic cortical neuron viability was confirmed under the thermal conditions produced by radiofrequency stimulation of iron oxide nanoparticles. While these results are not directly applicable to clinical applications of hyperthermia, the thermotolerance of chick embryonic cortical neurons is promising and calls for further studies employing human cultures of neurons and glial cells. © 2014 Informa UK Ltd.

Department(s)

Chemical and Biochemical Engineering

Publication Status

Free Access

Comments

National Science Foundation, Grant CBET 0846433

Keywords and Phrases

Aminosilane; Cortical neurons; Iron oxide nanoparticles; Superparamagnetic

International Standard Serial Number (ISSN)

1464-5157; 0265-6736

Document Type

Article - Journal

Document Version

Final Version

File Type

text

Language(s)

English

Rights

© 2025 The Authors, All rights reserved.

Publication Date

01 Jan 2014

PubMed ID

24471936

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