Abstract
Ion-exchange porous adsorbent media having intermediate and low surface densities of dextran polymer grafted on the surface of the through pores of polymeric monoliths are constructed and characterized by a molecular dynamic modeling and simulation approach that has also been shown to be effective in the construction and characterization of porous ion-exchange adsorbent media whose number of immobilized dextran polymer chains per unit surface area is high. the activation step that prepares the surface of the pores of the dextran polymer layer for the immobilization of the charged ligands insignificantly affected the pore structure of the dextran polymer layer, while this was found to not be the case for previously studied systems that involved high dextran polymer surface densities. Compared to the high dextran polymer density system studied previously, the intermediate dextran polymer density system can generate significantly larger pores but still possesses relatively high interconnection and mutual steric support between dextran chains to exhibit similar structural characteristics and responses to charged ligand immobilization, including dextran layer thickness, stability, monomer distribution, ligand-induced compact chain structures, dextran layer shrinkage, distributions of ligands and counterions, and local Non electroneutrality. the low dextran polymer density system having relatively isolated dextran chains and insufficient mutual steric support can result in even larger pores than those obtained in the intermediate dextran polymer density system, but a significantly thinner, porous dextran polymer layer and different dextran monomer distributions are obtained in the low dextran polymer density system. More importantly, the gradient of the local Non electroneutrality after the immobilization of the charged ligands is significantly smaller in magnitude in the low dextran polymer density system than that obtained in the system having intermediate dextran polymer density, and, despite a lack of porous layer depth to accommodate adsorbate biomolecules in large amounts, it could still be useful for the effective transport and adsorption of very large biomolecules.
Recommended Citation
E. Riccardi et al., "The Design by Molecular Dynamics Modeling and Simulations of Porous Polymer Adsorbent Media Immobilized on the throughpore Surfaces of Polymeric Monoliths," Journal of Chromatographic Science, vol. 47, no. 6, pp. 459 - 466, Oxford University Press, Jan 2009.
The definitive version is available at https://doi.org/10.1093/chromsci/47.6.459
Department(s)
Chemical and Biochemical Engineering
International Standard Serial Number (ISSN)
0021-9665
Document Type
Article - Journal
Document Version
Citation
File Type
text
Language(s)
English
Rights
© 2024 Oxford University Press, All rights reserved.
Publication Date
01 Jan 2009
