Purification of Human Antibodies by using Liquid/liquid Extraction

Abstract

Aqueous two-phase extraction is a well-established technique with wide spread use in laboratory separations and some impact in industrial scale separations. We have investigated the use of aqueous two phase systems for the purification of a human antibody expressed in corn. the antibody was expressed fully non-glycosylated. We show that by using a poly(ethylene)glycol/phosphate or poly(ethylene)glycol/citrate systems in combination with an interface precipitation step it is possible to recover 80 percent of the antibody with a 10-fold purity. the method is simple, very robust, and easy to scale up.

Department(s)

Chemical and Biochemical Engineering

Document Type

Article - Conference proceedings

Document Version

Citation

File Type

text

Language(s)

English

Rights

© 2024 American Institute of Chemical Engineers, All rights reserved.

Publication Date

01 Dec 2005

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