Abstract
Liquid/liquid partition chromatography is a mild yet powerful separation method for a variety of biological materials. This work demonstrates that it should be feasible to immobilize an ethylene oxide-propylene oxide (EO/PO) random copolymer solution and to use a solution of NaCl equilibrated against the polymer solution as the mobile-phase (poly (EO-PO) [P(EO-PO)] and NaCl form two aqueous phases known as aqueous two-phase systems). Three random copolymers with different molecular weights and EO/PO ratios were used. Dodecyl-agarose and polystyrene were tested as possible supports. The wetting energies of the aqueous two-phase systems on these two kinds of surfaces were calculated as well as contact angles for each phase on the same surfaces. Finally, the thickness of P(EO-PO) adsorption layers on polystyrene lattices were measured by dynamic light scattering. Contact angle measurements indicate that indeed some EO/PO copolymers preferentially wet hydrophobic substrates, forming thin films. © 2002 John Wiley & Sons, Inc.
Recommended Citation
Y. Huang and D. Forciniti, "Ethylene Oxide and Propylene Oxide Random Copolymer/sodium Chloride Aqueous Two-phase Systems: Wetting and Adsorption on Dodecyl-agarose and Polystyrene," Biotechnology and Bioengineering, vol. 77, no. 7, pp. 786 - 795, Wiley, Mar 2002.
The definitive version is available at https://doi.org/10.1002/bit.10169
Department(s)
Chemical and Biochemical Engineering
Publication Status
Full Access
Keywords and Phrases
Dodecyl-agarose; Ethylene oxide; Polystyrene; Propylene oxide
International Standard Serial Number (ISSN)
0006-3592
Document Type
Article - Journal
Document Version
Citation
File Type
text
Language(s)
English
Rights
© 2024 Wiley, All rights reserved.
Publication Date
30 Mar 2002
PubMed ID
11835139
