Experimental Composite Guidance Conduits for Peripheral Nerve Repair: An Evaluation of Ion Release

Author

X. F. Zhang
A. Coughlan
H. O'Shea
Mark R. Towler, Missouri University of Science and technologyFollow
S. Kehoe
D. Boyd

Abstract

Poly (lactide-co-glycolide) (PLGA) - Pluronic F127 - glass composites have demonstrated excellent potential, from the perspective of controlled mechanical properties and cytocompatibility, for peripheral nerve regeneration. In addition to controlling the mechanical properties and cytotoxicity for such composite devices, the glass component may mediate specific responses upon implantation via degradation in the physiological environment and release of constituent elements. However, research focused on quantifying the release levels of such therapeutic ions from these experimental medical devices has been limited. To redress the balance, this paper explores the ion release profiles for Si⁴⁺, Ca²⁺, Na⁺, Zn ²⁺, and Ce⁴⁺ from experimental composite nerve guidance conduits (CNGC) comprising PLGA (at 12.5, and 20 wt.%), F127 (at 0, 2.5 and 5 wt.%) and various loadings of Si-Ca-Na-Zn-Ce glass (at 20 and 40 wt.%) for incubation periods of up to 28 days. The concentration of each ion, at various time points, was determined using Inductively Coupled Plasma-Atomic Emission Spectrometry (Perkin Elmer Optima 3000). It was observed that the Si⁴⁺, Na⁺, Ca²⁺, Zn²⁺ release from CNGCs in this study ranged from 0.22 to 6.477 ppm, 2.307 to 3.277 ppm, 40 to 119 ppm, and 45 to 51 ppm, respectively. The Ce 4+ concentrations were under the minimum detection limits for the ICP instrument utilized. The results indicate that the ion release levels may be appropriate to mediate therapeutic effects with respect to peripheral nerve regeneration. The data generated in this paper provides requisite evidence to optimize composition for pre-clinical evaluation of the experimental composite. © 2012 Elsevier B.V.

Recommended Citation

X. F. Zhang et al., "Experimental Composite Guidance Conduits for Peripheral Nerve Repair: An Evaluation of Ion Release," Materials Science and Engineering C, vol. 32, no. 6, pp. 1654 - 1663, Elsevier, Aug 2012.

The definitive version is available at https://doi.org/10.1016/j.msec.2012.04.058

Department(s)

Chemical and Biochemical Engineering

International Standard Serial Number (ISSN)

0928-4931

Document Type

Article - Journal

Document Version

Final Version

File Type

text

Language(s)

English

Rights

© 2023 Elsevier, All rights reserved.

Publication Date

01 Aug 2012

PubMed ID

24364973