Abstract
Glass polyalkenoate cements (GPCs) are under investigation as potential bone adhesives, as they may provide an alternative to polymethylmethacrylate-based cements. GPCs containing strontium (Sr) and zinc (Zn) in place of aluminum (Al) are of particular interest because these ions are known stimulators of osteoprogenitor differentiation. GPCs have been manufactured from a novel bioactive glass (SiO2:0.48, ZnO:0.36, CaO:0.12, SrO:0.04) in the past, but, while such materials have been assessed for their influence on viability, their influence on osteogenic function has not been investigated until now. For this study, two GPCs were formulated from the same glass precursor evaluated in previous studies. These GPCs were named GPC A and GPC B, and they differed in glass particle size, polyacrylic acid molecular weight, and their powder: liquid ratios. The effect of these two GPCs on osteogenic differentiation of primary rat osteoblasts were evaluated using three culture systems: culture with dissolution extracts, indirect contact with trans well-inserts and direct contact. Additionally, the degradation characteristics of GPCs were assessed, including their interfacial pH and surrounding pH. The experimental outcomes revealed that collagen deposition, alkaline phosphatase expression, and mineralization were largely dependent on GPC composition as well as the mode of interaction with cells. These markers were found to be significantly elevated in response to GPC A's dissolution products. However, osteogenic differentiation was inhibited when osteoblasts were cultured indirectly and directly with GPCs, with, overall, GPC B significantly outperforming GPC A. These results suggest that GPC degradation products effect osteogenic differentiation in a dose-dependent manner.
Recommended Citation
D. Marx et al., "In Vitro Osteogenic Performance of Two Novel Strontium and Zinc-Containing Glass Polyalkenoate Cements," Journal of Biomedical Materials Research - Part A, vol. 109, no. 8, pp. 1366 - 1378, Wiley, Aug 2021.
The definitive version is available at https://doi.org/10.1002/jbm.a.37127
Department(s)
Chemical and Biochemical Engineering
International Standard Serial Number (ISSN)
1552-4965; 1549-3296
Document Type
Article - Journal
Document Version
Final Version
File Type
text
Language(s)
English
Rights
© 2023 Wiley, All rights reserved.
Publication Date
01 Aug 2021
PubMed ID
33125181
Included in
Biochemical and Biomolecular Engineering Commons, Biomedical Devices and Instrumentation Commons
Comments
Canadian Institutes of Health Research, Grant 399463