Abstract
While it is widely acknowledged that the ubiquitin-proteasome system plays an important role in transcription, little is known concerning the mechanistic basis, in particular the spatial organization of proteasome-dependent proteolysis at the transcription site. Here, we show that proteasomal activity and tetraubiquitinated proteins concentrate to nucleoplasmic microenvironments in the euchromatin. Such proteolytic domains are immobile and distinctly positioned in relation to transcriptional processes. Analysis of gene arrays and early genes in Caenorhabditis elegans embryos reveals that proteasomes and proteasomal activity are distantly located relative to transcriptionally active genes. In contrast, transcriptional inhibition generally induces local overlap of proteolytic microdomains with components of the transcription machinery and degradation of RNA polymerase II. The results establish that spatial organization of proteasomal activity differs with respect to distinct phases of the transcription cycle in at least some genes, and thus might contribute to the plasticity of gene expression in response to environmental stimuli. © 2011 The Author(s).
Recommended Citation
A. Scharf et al., "Distant Positioning Of Proteasomal Proteolysis Relative To Actively Transcribed Genes," Nucleic Acids Research, vol. 39, no. 11, pp. 4612 - 4627, Oxford University Press, Jun 2011.
The definitive version is available at https://doi.org/10.1093/nar/gkr069
Department(s)
Biological Sciences
Publication Status
Open Access
International Standard Serial Number (ISSN)
1362-4962; 0305-1048
Document Type
Article - Journal
Document Version
Final Version
File Type
text
Language(s)
English
Rights
© 2023 Oxford University Press, All rights reserved.
Publication Date
01 Jun 2011
PubMed ID
21306993
Comments
National Institutes of Health, Grant R01HL079566