Isochronal Visualization Of Transcription And Proteasomal Proteolysis In Cell Culture Or In The Model Organism, Caenorhabditis Elegans
Abstract
Investigation of differential gene regulation by protein degradation requires analysis of the spatial and temporal association between proteolysis and transcription. Here, we describe the isochronal visualization of proteasomal proteolysis and transcription in cell culture or in vivo in the model organism Caenorhabditis elegans. This includes localization of proteasome-dependent proteolysis by fluorescent degradation products of model and endogenous substrates of the proteasome in combination with immunolabelling of RNA polymerase II and transcription in situ run-on assays. © 2013 Springer Science+Business Media, LLC.
Recommended Citation
A. Von Mikecz and A. Scharf, "Isochronal Visualization Of Transcription And Proteasomal Proteolysis In Cell Culture Or In The Model Organism, Caenorhabditis Elegans," Methods in Molecular Biology, vol. 1042, pp. 257 - 273, Springer, Jan 2013.
The definitive version is available at https://doi.org/10.1007/978-1-62703-526-2_19
Department(s)
Biological Sciences
Keywords and Phrases
Cell nucleus; In situ run-on assay; K48; Microinjection; Proteasome; Proteolysis; RNA polymerase II; Transcription; Ubiquitination
International Standard Book Number (ISBN)
978-162703525-5
International Standard Serial Number (ISSN)
1064-3745
Document Type
Article - Journal
Document Version
Citation
File Type
text
Language(s)
English
Rights
© 2023 Springer, All rights reserved.
Publication Date
01 Jan 2013
PubMed ID
23980014
