Abstract
Mobile DNA elements play a significant evolutionary role by promoting genome plasticity. Insertion sequences are the smallest prokaryotic transposable elements. They are highly diverse elements, and the ability to accurately identify, annotate, and infer the full genomic impact of insertion sequences is lacking. Halanaerobium hydrogeniformans is a haloalkaliphilic bacterium with an abnormally high number of insertion sequences. One family, IS200/IS605, showed several interesting features distinct from other elements in this genome. Twenty-three loci harbor elements of this family in varying stages of decay, from nearly intact to an ends-only sequence. The loci were characterized with respect to two divergent open reading frames (ORF), tnpA and tnpB, and left and right ends of the elements. The tnpB ORF contains two nearly identical insert sequences that suggest recombination between tnpB ORF is occurring. From these results, insertion sequence activity can be inferred, including transposition capability and element interaction.
Recommended Citation
M. Sadler et al., "Characterization of the IS200/IS605 Insertion Sequence Family in Halanaerobium Hydrogeniformans," Genes, vol. 11, no. 5, MDPI AG, May 2020.
The definitive version is available at https://doi.org/10.3390/genes11050484
Department(s)
Biological Sciences
Research Center/Lab(s)
Center for Research in Energy and Environment (CREE)
Keywords and Phrases
Extremophilic bacteria; Insertion sequences; IS200/605; Mobile DNA; Transposable elements
International Standard Serial Number (ISSN)
2073-4425; 2073-4425
Document Type
Article - Journal
Document Version
Final Version
File Type
text
Language(s)
English
Rights
© 2020 The Authors, All rights reserved.
Creative Commons Licensing
This work is licensed under a Creative Commons Attribution 4.0 License.
Publication Date
01 May 2020
