Sequence and Structure of a Phenylalanine Ammonia-Lyase Gene from Glycine Max

Abstract

The gene encoding a key enzyme in anthocyanin biosynthesis, phenylalanine ammonia-lyase (PAL), was cloned from soybean (Glycine max). The purpose was to obtain a molecular probe to study the organization of this gene family in soybean and to examine novel regulatory mechanisms present in the anthocyanin biosynthetic pathway of this system. A soybean genomic library was constructed in the bacteriophage vector lambda Charon 35. A PAL cDNA clone from Phaseolus vulgaris was used in screening the library, and two PAL genes were isolated. One gene was sequenced entirely and analyzed by sequence homology to the PAL2 gene of Phaseolus vulgaris. Genomic analysis indicates that PAL sequences of Glycine max exist as a small gene family consisting of only two to three members. The representative PAL gene sequenced (PAL1) has a coding region of 2142 basepairs divided among two exons. The single intron is 1519 basepairs and splits the 131st codon.

Department(s)

Biological Sciences

Keywords and Phrases

Anthocyanin Biosynthesis; Flavonoids; General Phenylpropanoid Metabolism; PAL Gene; Soybean

International Standard Serial Number (ISSN)

1940-1744;1940-1744

Document Type

Article - Journal

Document Version

Citation

File Type

text

Language(s)

English

Rights

© 1991 Informa Healthcare, All rights reserved.

Publication Date

01 Jan 1991

PubMed ID

1799682

Link to Full Text

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