Microsatellite Primers for the Atlantic Coastal Killifish, Fundulus Heteroclitus, with Applicability to Related Fundulus Species

Abstract

The mummichog (Fundulus heteroclitus), a common Atlantic coastal killifish, is a model vertebrate species for the study of molecular genetic variation in natural populations and of environmental toxicology. We report the development of a set of 20 microsatellite loci in this species. Average expected heterozygosity across all loci was 0.84 (range: 0.60-0.97), revealing a high level of variability at most loci. A survey of seven additional Fundulus species yielded one or two robust amplification products in over half (63%) of the species-primer combinations tested. Therefore, many of these loci will also prove useful in studies of other members of the genus Fundulus.

Department(s)

Biological Sciences

Keywords and Phrases

Cyprinodontiformes; Fundulus; Fundulus catenatus; Fundulus cingulatus; Fundulus confluentus; Fundulus heteroclitus; Fundulus majalis; Fundulus olivaceus; Fundulus seminolis; Fundulus similis; Vertebrata

International Standard Serial Number (ISSN)

1471-8278

Document Type

Article - Journal

Document Version

Citation

File Type

text

Language(s)

English

Rights

© 2005 Blackwell Publishing, All rights reserved.

Publication Date

01 Feb 2005

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