Isolation and Characterization of Microsatellite Loci for the Blackstripe Topminnow Fundulus Notatus and Their Variability in Two Closely Related Species
A total of 17 polymorphic microsatellite loci were isolated from the blackstripe topminnow Fundulus notatus. In a sample of 29 individuals, these loci were found to possess two to 19 alleles with expected heterozygosity values ranging from 0·212 to 0·919 and all but one of the loci conformed to Hardy–Weinberg equilibrium expectations. Many of these loci were polymorphic in the closely related species Fundulus olivaceus and Fundulus euryzonus providing a set of markers that should prove useful in future ecological and evolutionary studies of members of this species complex.
K. A. Feldheim et al., "Isolation and Characterization of Microsatellite Loci for the Blackstripe Topminnow Fundulus Notatus and Their Variability in Two Closely Related Species," Journal of Fish Biology, vol. 85, no. 5, pp. 1726-1732, Blackwell Publishing Ltd, Aug 2014.
The definitive version is available at https://doi.org/10.1111/jfb.12469
Keywords and Phrases
Fundulidae; Fundulus notatus; microsatellite DNA; allele; animal; Cypriniformes; DNA sequence; gene locus; genetics; Alleles; Animals; Cyprinidae; Genetic Loci; Microsatellite Repeats; Sequence Analysis; DNA; Cross-species amplification; Fundulidae; Genetic marker; Population genetics; Primer sequence
International Standard Serial Number (ISSN)
Article - Journal
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01 Aug 2014