Isolation and Characterization of Microsatellite Loci for the Blackstripe Topminnow Fundulus Notatus and Their Variability in Two Closely Related Species

Abstract

A total of 17 polymorphic microsatellite loci were isolated from the blackstripe topminnow Fundulus notatus. In a sample of 29 individuals, these loci were found to possess two to 19 alleles with expected heterozygosity values ranging from 0·212 to 0·919 and all but one of the loci conformed to Hardy–Weinberg equilibrium expectations. Many of these loci were polymorphic in the closely related species Fundulus olivaceus and Fundulus euryzonus providing a set of markers that should prove useful in future ecological and evolutionary studies of members of this species complex.

Department(s)

Biological Sciences

Keywords and Phrases

Fundulidae; Fundulus notatus; microsatellite DNA; allele; animal; Cypriniformes; DNA sequence; gene locus; genetics; Alleles; Animals; Cyprinidae; Genetic Loci; Microsatellite Repeats; Sequence Analysis; DNA; Cross-species amplification; Fundulidae; Genetic marker; Population genetics; Primer sequence

International Standard Serial Number (ISSN)

0022-1112

Document Type

Article - Journal

Document Version

Citation

File Type

text

Language(s)

English

Rights

© 2014 Blackwell Publishing Ltd, All rights reserved.

Publication Date

01 Aug 2014

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