"The enzyme indole acetic acid oxidase is produced by the fungus Hygrophorous conicus when it is grown in the presence of indole acetic acid (IAA) or tryptamine. The IAA acts both as an inducer and a substrate for this intracellular enzyme. The product of the oxidation catalyzed by the IAA oxidase is oxindole acetic acid (OIAA).
The rate of growth and the rate of formation of the enzyme IAA oxidase by H.conicus were studied in different media. Of these, a nitrate medium containing 0.5 per cent Yeast extract (Y.E.) was found to be the best for the production of H.conicus mycelium. A maltose medium was better than the other media tested so far as the growth of H,conicus and the formation of the enzyme IAA oxidase were concerned, The addition of 0.5 per cent Y.E. to any of the media tested suppressed the formation of the enzyme IAA oxidase of H.conicus.
The Krebs cycle enzyme succinic dehydrogenase was found in the particulate fraction, sedimented at 24,000 x g for 20 minutes from a cell-free preparation of H,conicus.
The use of sonic oscillations for 10 minutes at 4-5⁰C was found to solubilize, to some extent, the enzyme succinic dehydro genase of H,conicus.
The phenazine methosulfate method for the assay of succinic dehydrogenase activity gave higher QO₂(N) values than the cytochrome c method"--Abstract, pages ii-iii.
Siehr, Donald J.
Grigoropoulos, Sotirios G.
Kincannon, Donny F.
Hanna, Samir B.
M.S. in Chemistry
University of Missouri at Rolla
viii, 90 pages
© 1966 Jagdish M. Mehta, All rights reserved.
Thesis - Open Access
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Mehta, Jagdish M., "The activity of succinic dehydrogenase and indole acetic acid oxidase in cell-free preparations of Hygrophorous conicus" (1966). Masters Theses. 5728.