"The objectives of this investigation were: (a) to culture large quantities of Thiobacillus thiooxidans cells, (b) to find an acceptable method of rupturing the cells, (c) to find an acceptable method of isolating the cell walls, and (d) to analyze the cell wall components, especially the amino acid and sugar components. The strain of T. thiooxidans used for this investigation was American Type Culture Collection Number 8085. The surface active agents Tergitol 08 and Tween 80 were investigated for their ability to reduce the lag period found in the growth of T. thiooxidans. Although a concentration of 12.5 ppm Tween 80 did decrease the lag period, the use of these surfactants was not effective in increasing the amount of cells grown over a specific time period because they reduced the cell yield. Two cell rupture techniques, sonication and pressure cell rupture, coupled with either washing with deionized water or washing treatment with IR-120 and AG-1X2 ion exchange resins, were tested"--Abstract, page ii-iii.
Siehr, Donald J.
Peterson, John E.
Grigoropoulos, Sotirios G.
Grice, Harvey H., 1912-1993
Wellek, Robert M.
Chemical and Biochemical Engineering
Ph. D. in Chemical Engineering
University of Missouri at Rolla
ix, 102 pages
© 1967 Edward Hibbert Crum, All rights reserved.
Dissertation - Open Access
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Crum, Edward Hibbert, "The amino acid and monosaccharide content of the cell walls of Thiobacillus thiooxidans" (1967). Doctoral Dissertations. 1874.