Title

Biodiversity Assessment in Ozark Streams Using eDNA

Presenter Information

Jessica Newbury

Department

Biological Sciences

Major

Biological Sciences

Research Advisor

Duvernell, David D. (David Douglas), 1970-

Advisor's Department

Biological Sciences

Funding Source

Professor funded

Abstract

Environmental DNA (eDNA) methods of biodiversity sampling have become well documented, utilized and depended upon as a source of data, using DNA generated by epithelial cell deposition and then using PCR restriction fragment length polymorphism markers to distinguish between species. Despite the surge of research data generated via sampling eDNA, technical challenges still facing the usage of eDNA today include verifying that eDNA samples recovered accurately represent the biotic community and exploring the extent that technical errors can affect sampling results, acknowledging the power of PCR sampling and the challenge it poses to eliminating contamination. In this project, we aspire to validate this (eDNA) technique by: (1) collecting eDNA data by sampling three different stream sites and comparing the results to traditional biodiversity samples collected previously and extensively by the regional Missouri Department of Conservation office for our specific local stream habitats and (2) devising positive and negative controls that can be applied to monitor for contamination issues.

Biography

Jessica Newburry is currently a junior at S&T. She has been a part of three reasearch teams, including a Neuroscience research team, a water Ecology research team, and a Population Genetics team currently. In her spare time, she enjoys reading, painting, and working with animals, including her dog, Shadow. She also enjoys spending time with her fiancé, Chandler Mossman, a fellow Biological Sciences major. She aspires to achieve a PhD, hoping to secure a career in conservation in Alaska.

Presentation Type

OURE Fellows Proposal Oral Applicant

Document Type

Presentation

Location

Missouri Room

Presentation Date

16 Apr 2019, 2:00 pm - 2:30 pm

This document is currently not available here.

Share

COinS
 
Apr 16th, 2:00 PM Apr 16th, 2:30 PM

Biodiversity Assessment in Ozark Streams Using eDNA

Missouri Room

Environmental DNA (eDNA) methods of biodiversity sampling have become well documented, utilized and depended upon as a source of data, using DNA generated by epithelial cell deposition and then using PCR restriction fragment length polymorphism markers to distinguish between species. Despite the surge of research data generated via sampling eDNA, technical challenges still facing the usage of eDNA today include verifying that eDNA samples recovered accurately represent the biotic community and exploring the extent that technical errors can affect sampling results, acknowledging the power of PCR sampling and the challenge it poses to eliminating contamination. In this project, we aspire to validate this (eDNA) technique by: (1) collecting eDNA data by sampling three different stream sites and comparing the results to traditional biodiversity samples collected previously and extensively by the regional Missouri Department of Conservation office for our specific local stream habitats and (2) devising positive and negative controls that can be applied to monitor for contamination issues.