Traumatic Brain Injury Diagnosis Via HPLC-MS/MS
Department
Chemistry
Major
Biochemistry
Research Advisor
Shi, Honglan
Advisor's Department
Chemistry
Funding Source
OURE Fellows Program, Leanard Wood Institute
Abstract
Over 2.5 million cases of traumatic brain injury (TBI) are reported each year in the United States and are involved in 30% of all injury-related deaths. TBl's can occur in a variety of ways and are thus difficult to diagnose. Current methods either include extensive imaging using expensive equipment or simple qualitative assessments that analyze a patient's verbal and motor skill. Due to the lack of an efficient method of TBI characterization, a metabolomics approach will be taken in order to design a biomarker profile for the complex injury. By utilizing high-performance liquid chromatography - tandem mass spectroscopy (HPLC - MS/MS), a quantitative method was designed to analyze the concentrations of eight compounds in urine that have been previously linked with traumatic brain injury. This method was then used to analyze the urine of students, to prove method efficacy in real urine samples and establish the average concentrations of these metabolites in urine.
Biography
Alex is a senior chemistry student and has been conducting research in the chemistry department since his freshman year. Throughout his time at S& T, he has been an active member of the chemistry department and has been involved in numerous projects across several disciplines of chemistry. Alex will graduate this May and is currently seeking work in analytical chemistry in St. Louis.
Presentation Type
OURE Fellows Final Oral Presentation
Document Type
Presentation
Location
Missouri Room
Presentation Date
16 Apr 2019, 9:30 am - 10:00 am
Traumatic Brain Injury Diagnosis Via HPLC-MS/MS
Missouri Room
Over 2.5 million cases of traumatic brain injury (TBI) are reported each year in the United States and are involved in 30% of all injury-related deaths. TBl's can occur in a variety of ways and are thus difficult to diagnose. Current methods either include extensive imaging using expensive equipment or simple qualitative assessments that analyze a patient's verbal and motor skill. Due to the lack of an efficient method of TBI characterization, a metabolomics approach will be taken in order to design a biomarker profile for the complex injury. By utilizing high-performance liquid chromatography - tandem mass spectroscopy (HPLC - MS/MS), a quantitative method was designed to analyze the concentrations of eight compounds in urine that have been previously linked with traumatic brain injury. This method was then used to analyze the urine of students, to prove method efficacy in real urine samples and establish the average concentrations of these metabolites in urine.
