Title

BTree: A Bacillus Thuringiensis Toxin Targeting EABs

Presenter Information

Ryan Baumann

Department

Biological Sciences

Major

Biological Sciences

Research Advisor

Westenberg, David J.

Advisor's Department

Biological Sciences

Funding Source

Missouri S&T Biology, Chemical Engineering and SDELC

Abstract

Since the year 2002, North American ash trees have been infected with and killed by an invasive beetle species known as Emerald Ash Borers (EAB). Current methods for prevention and treatment of EAB’s are expensive and inadequate. Our proposed long term solution is to develop Ash trees that are genetically resistant to EAB’s. From a known Bacillus thuringiensis Cry8Da protein, we hope to induce mutations in the protein’s receptor binding regions to create a Bt toxin specific for EAB’s. After screening modified proteins, we will utilize leaf-specific expression of the Cry Toxin in Arabidopsis thaliana as our model system for Ash trees. We hope to present this system for future development as a safe and effective alternative to current treatment methods used in affected areas.

Biography

Ryan Baumann is a Senior in Biological Sciences from Saint Louis, MO. He has spent all 8 semesters a member of the Missouri S&T International Genetically Engineered Machine (iGEM) Team. He has served as the Vice President, Lab Manager, and has assisted in the design and lab work of three individual year long projects. He is a Student Ambassador in the Missouri S&T Admissions Office, has worked two summer internships, and has served positions in the Helix Life Sciences Club.

Presentation Type

OURE Fellows Final Oral Presentation

Document Type

Presentation

Location

Missouri Room

Presentation Date

16 Apr 2019, 9:00 am - 9:30 am

Comments

Joint Project in conjunction with the Missouri S&T iGEM Team

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Apr 16th, 9:00 AM Apr 16th, 9:30 AM

BTree: A Bacillus Thuringiensis Toxin Targeting EABs

Missouri Room

Since the year 2002, North American ash trees have been infected with and killed by an invasive beetle species known as Emerald Ash Borers (EAB). Current methods for prevention and treatment of EAB’s are expensive and inadequate. Our proposed long term solution is to develop Ash trees that are genetically resistant to EAB’s. From a known Bacillus thuringiensis Cry8Da protein, we hope to induce mutations in the protein’s receptor binding regions to create a Bt toxin specific for EAB’s. After screening modified proteins, we will utilize leaf-specific expression of the Cry Toxin in Arabidopsis thaliana as our model system for Ash trees. We hope to present this system for future development as a safe and effective alternative to current treatment methods used in affected areas.