Transposable Elements in Soap Lake's Halomonas #7
Department
Biological Sciences
Major
Biology
Research Advisor
Frank, Ronald L.
Advisor's Department
Biological Sciences
Funding Source
OURE
Abstract
Transposable elements are DNA sequences that can move or copy themselves within a genome. As a result insertional mutations can be reversed,gained, and genomes expanded. Bacterial genomes consist mainly of protein-coding regions and therefore bacterial transposable elements can have a significant impact on these small genomes. The transposable elements of Soap Lake s Halomonas # 7 were examined in this research. Our analysis started with an algorithmic annotation of the genome to locate transposable elements. We used BLAST (Basic Local Alignment Search Tool) at NCBI (National Center for Biotechnology Information) to search the genome for matches to the initial sequence. Once the left and right ends of each element were approximated we identified inverted repeats for each element that are characteristic of these families. Using the inverted repeats we identified target site duplications and identified the start and stop codons of the open reading frames that encode the transposase enzyme required for copying elements. We were able to locate, identify, and characterize four different transposable element families with multiple copies in the genome.
Biography
Madison Hogan is a junior in the Biology department at Missouri University of Science and Technology. She attended classes for two years at East Central College with the assistance of the A+ Program from tutoring the special education classes at Rolla Senior Highschool. Some of her notable position including working as a natural resource specialist as Onondaga Cave State Park and holding the position of vice President and President of Phi Theta Kappa's beta omicorn phi chapter at East Central College's Rolla location. Madison is using this research opportunity to further her experience with different aspects of the genetics field.
Research Category
Sciences
Presentation Type
Poster Presentation
Document Type
Poster
Location
Upper Atrium
Presentation Date
16 Apr 2019, 9:00 am - 3:00 pm
Transposable Elements in Soap Lake's Halomonas #7
Upper Atrium
Transposable elements are DNA sequences that can move or copy themselves within a genome. As a result insertional mutations can be reversed,gained, and genomes expanded. Bacterial genomes consist mainly of protein-coding regions and therefore bacterial transposable elements can have a significant impact on these small genomes. The transposable elements of Soap Lake s Halomonas # 7 were examined in this research. Our analysis started with an algorithmic annotation of the genome to locate transposable elements. We used BLAST (Basic Local Alignment Search Tool) at NCBI (National Center for Biotechnology Information) to search the genome for matches to the initial sequence. Once the left and right ends of each element were approximated we identified inverted repeats for each element that are characteristic of these families. Using the inverted repeats we identified target site duplications and identified the start and stop codons of the open reading frames that encode the transposase enzyme required for copying elements. We were able to locate, identify, and characterize four different transposable element families with multiple copies in the genome.
