Transfer of Bacterial Genome into Yeast
Department
Biological Sciences
Major
Chemical Engineering
Research Advisor
Shannon, Katie
Advisor's Department
Biological Sciences
Abstract
The transfer of a genome from bacteria to budding yeast enables genomic engineering for those bacteria that are difficult to culture in the lab and lack tools and protocols for genetic manipulation. Budding yeast Saccharomyces cerevisiae provide the advantage of simple and effective genetic tools for transfer and manipulation of DNA. This project’s goal is to transfer the genome from the Halanaerobium hydrogeniformans into a yeast cell in order to allow for genetic manipulation of the bacterial genome. For this to be possible, a plasmid needs to be created that contains a sequence that allows for the transfer of the DNA into bacteria, a selectable marker for yeast, a yeast centromere sequence, and a yeast replication origin. Then, the plasmid can be added to the bacteria, and integrated into its genome. If the plasmid is successfully incorporated into the bacterial genome, the resulting genome can be transferred to yeast cells. This will later allow for engineering of the bacterial genome using mutations.
Biography
Stephanie Kroger is a currently a sophomore at Missouri University of Science and Technology. She has always been interested in biology, and after taking a Cell Biology class in the fall, she joined Dr. Shannon’s lab in the spring semester of 2015. She has learned a lot in the short time that she has been working in the lab, and enjoys learning more each day. She will continue her education and follow her love of biology to earn a degree in Biomedical Engineering. She hopes to be a part of the forefront to develop new technologies and advancements in the medical field.
Research Category
Sciences
Presentation Type
Poster Presentation
Document Type
Poster
Location
Upper Atrium/Hall
Presentation Date
15 Apr 2015, 9:00 am - 11:45 am
Transfer of Bacterial Genome into Yeast
Upper Atrium/Hall
The transfer of a genome from bacteria to budding yeast enables genomic engineering for those bacteria that are difficult to culture in the lab and lack tools and protocols for genetic manipulation. Budding yeast Saccharomyces cerevisiae provide the advantage of simple and effective genetic tools for transfer and manipulation of DNA. This project’s goal is to transfer the genome from the Halanaerobium hydrogeniformans into a yeast cell in order to allow for genetic manipulation of the bacterial genome. For this to be possible, a plasmid needs to be created that contains a sequence that allows for the transfer of the DNA into bacteria, a selectable marker for yeast, a yeast centromere sequence, and a yeast replication origin. Then, the plasmid can be added to the bacteria, and integrated into its genome. If the plasmid is successfully incorporated into the bacterial genome, the resulting genome can be transferred to yeast cells. This will later allow for engineering of the bacterial genome using mutations.
