Title

Transfer of Bacterial Genome into Yeast

Presenter Information

Stephanie Kroger

Department

Biological Sciences

Major

Chemical Engineering

Research Advisor

Shannon, Katie

Advisor's Department

Biological Sciences

Abstract

The transfer of a genome from bacteria to budding yeast enables genomic engineering for those bacteria that are difficult to culture in the lab and lack tools and protocols for genetic manipulation. Budding yeast Saccharomyces cerevisiae provide the advantage of simple and effective genetic tools for transfer and manipulation of DNA. This project’s goal is to transfer the genome from the Halanaerobium hydrogeniformans into a yeast cell in order to allow for genetic manipulation of the bacterial genome. For this to be possible, a plasmid needs to be created that contains a sequence that allows for the transfer of the DNA into bacteria, a selectable marker for yeast, a yeast centromere sequence, and a yeast replication origin. Then, the plasmid can be added to the bacteria, and integrated into its genome. If the plasmid is successfully incorporated into the bacterial genome, the resulting genome can be transferred to yeast cells. This will later allow for engineering of the bacterial genome using mutations.

Biography

Stephanie Kroger is a currently a sophomore at Missouri University of Science and Technology. She has always been interested in biology, and after taking a Cell Biology class in the fall, she joined Dr. Shannon’s lab in the spring semester of 2015. She has learned a lot in the short time that she has been working in the lab, and enjoys learning more each day. She will continue her education and follow her love of biology to earn a degree in Biomedical Engineering. She hopes to be a part of the forefront to develop new technologies and advancements in the medical field.

Research Category

Sciences

Presentation Type

Poster Presentation

Document Type

Poster

Location

Upper Atrium/Hall

Presentation Date

15 Apr 2015, 9:00 am - 11:45 am

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Apr 15th, 9:00 AM Apr 15th, 11:45 AM

Transfer of Bacterial Genome into Yeast

Upper Atrium/Hall

The transfer of a genome from bacteria to budding yeast enables genomic engineering for those bacteria that are difficult to culture in the lab and lack tools and protocols for genetic manipulation. Budding yeast Saccharomyces cerevisiae provide the advantage of simple and effective genetic tools for transfer and manipulation of DNA. This project’s goal is to transfer the genome from the Halanaerobium hydrogeniformans into a yeast cell in order to allow for genetic manipulation of the bacterial genome. For this to be possible, a plasmid needs to be created that contains a sequence that allows for the transfer of the DNA into bacteria, a selectable marker for yeast, a yeast centromere sequence, and a yeast replication origin. Then, the plasmid can be added to the bacteria, and integrated into its genome. If the plasmid is successfully incorporated into the bacterial genome, the resulting genome can be transferred to yeast cells. This will later allow for engineering of the bacterial genome using mutations.