Title

A Rapid, High-Performance Capillary Electrophoresis Method for Urinary Modified Nucleosides for Early Prostate Cancer Detection

Presenter Information

Sunghee Briana Choi

Department

Chemistry

Major

Chemistry

Research Advisor

Ma, Yinfa

Advisor's Department

Chemistry

Funding Source

Opportunities for Undergraduate Research Experiences (OURE)

Abstract

This project will expand on a recent paper (Jiang, Ma, 2009) in which a novel method (High-Performance Capillary Electrophoresis) was developed that separated and quantified nucleosides with high resolution. HPCE was used to target ten nucleosides previously implicated in prostate cancer (adenosine, xanthosine, guanosine, N2-methylguanosine, uridine, inosine, cytidine, 5-methylcytidine, 5-methyluridine, and 5-bromouridine). Various conditions in determining the appropriate buffer and concentration, separation voltage, and pH was identified to produce optimum separation of the analytes. Following the method development, reproducibility and sensitivity will be validated to substantiate any essential data relating to prostate cancer. Once optimal resolution and separation of the ten analytes are achieve, ~20 urine samples will be analyzed with the newly developed HPCE method.

Biography

Briana Choi is in her third year at Missouri S&T majoring in Chemistry with a Pre-Med emphasis. She was interested in getting a hands-on, learning experience with a research field she found interest in and get into research that could help her future career. She joined Dr. Ma’s research group last year and is hoping to further her learning experience at Missouri S&T.

Research Category

Sciences

Presentation Type

Poster Presentation

Document Type

Poster

Location

Upper Atrium/Hall

Presentation Date

15 Apr 2015, 9:00 am - 11:45 am

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Apr 15th, 9:00 AM Apr 15th, 11:45 AM

A Rapid, High-Performance Capillary Electrophoresis Method for Urinary Modified Nucleosides for Early Prostate Cancer Detection

Upper Atrium/Hall

This project will expand on a recent paper (Jiang, Ma, 2009) in which a novel method (High-Performance Capillary Electrophoresis) was developed that separated and quantified nucleosides with high resolution. HPCE was used to target ten nucleosides previously implicated in prostate cancer (adenosine, xanthosine, guanosine, N2-methylguanosine, uridine, inosine, cytidine, 5-methylcytidine, 5-methyluridine, and 5-bromouridine). Various conditions in determining the appropriate buffer and concentration, separation voltage, and pH was identified to produce optimum separation of the analytes. Following the method development, reproducibility and sensitivity will be validated to substantiate any essential data relating to prostate cancer. Once optimal resolution and separation of the ten analytes are achieve, ~20 urine samples will be analyzed with the newly developed HPCE method.