A Rapid, High-Performance Capillary Electrophoresis Method for Urinary Modified Nucleosides for Early Prostate Cancer Detection
Department
Chemistry
Major
Chemistry
Research Advisor
Ma, Yinfa
Advisor's Department
Chemistry
Funding Source
Opportunities for Undergraduate Research Experiences (OURE)
Abstract
This project will expand on a recent paper (Jiang, Ma, 2009) in which a novel method (High-Performance Capillary Electrophoresis) was developed that separated and quantified nucleosides with high resolution. HPCE was used to target ten nucleosides previously implicated in prostate cancer (adenosine, xanthosine, guanosine, N2-methylguanosine, uridine, inosine, cytidine, 5-methylcytidine, 5-methyluridine, and 5-bromouridine). Various conditions in determining the appropriate buffer and concentration, separation voltage, and pH was identified to produce optimum separation of the analytes. Following the method development, reproducibility and sensitivity will be validated to substantiate any essential data relating to prostate cancer. Once optimal resolution and separation of the ten analytes are achieve, ~20 urine samples will be analyzed with the newly developed HPCE method.
Biography
Briana Choi is in her third year at Missouri S&T majoring in Chemistry with a Pre-Med emphasis. She was interested in getting a hands-on, learning experience with a research field she found interest in and get into research that could help her future career. She joined Dr. Ma’s research group last year and is hoping to further her learning experience at Missouri S&T.
Research Category
Sciences
Presentation Type
Poster Presentation
Document Type
Poster
Location
Upper Atrium/Hall
Presentation Date
15 Apr 2015, 9:00 am - 11:45 am
A Rapid, High-Performance Capillary Electrophoresis Method for Urinary Modified Nucleosides for Early Prostate Cancer Detection
Upper Atrium/Hall
This project will expand on a recent paper (Jiang, Ma, 2009) in which a novel method (High-Performance Capillary Electrophoresis) was developed that separated and quantified nucleosides with high resolution. HPCE was used to target ten nucleosides previously implicated in prostate cancer (adenosine, xanthosine, guanosine, N2-methylguanosine, uridine, inosine, cytidine, 5-methylcytidine, 5-methyluridine, and 5-bromouridine). Various conditions in determining the appropriate buffer and concentration, separation voltage, and pH was identified to produce optimum separation of the analytes. Following the method development, reproducibility and sensitivity will be validated to substantiate any essential data relating to prostate cancer. Once optimal resolution and separation of the ten analytes are achieve, ~20 urine samples will be analyzed with the newly developed HPCE method.
