Analysis of Cellular Secretion via NMR and Toroid Cavity Probes
Department
Chemistry
Major
Biological Sciences
Research Advisor
Woelk, Klaus
Gerald, Rex
Advisor's Department
Chemistry
Funding Source
Missouri S&T’s Opportunities for Undergraduate Research Experiences (OURE) Program
Abstract
Yeast cells, such as Saccharomyces Cerevisiae, provide information for medical diagnostics, genetics, biochemical and microbiological applications. Due to structural, functional, and genetic similarities with higher level eukaryotic and human cells, yeast cells are common model cells. Yeast cells (3-4 μm in size) and their enzyme secretions should offer spectroscopic and spatial signatures via NMR and MRI. Initial experiment designs required the central conductor of a toroid cavity device (TCD) probe to be dip-coated in agar, exposed to yeast cells, and placed in incubation. After subsequent incubation periods, the central conductor was placed in a cylindrical TCD modified for insertion of the central conductor. Analysis utilized high field NMR spectrometers, and provided spectral information about the growth medium, secretions, and cells. The development of these new spectroscopic and imaging techniques for studies of cell secretion could be translated to the analysis of cancer cells.
Biography
Sierra Herndon is an incoming Freshman studying Biological Sciences at the University of Missouri Science and Technology. She has been working with Dr. Woelk’s and Dr. Gerald’s research group since August 2014 with the Nuclear Magnetic Resonance spectrometers and electronics.
Research Category
Research Proposals
Presentation Type
Poster Presentation
Document Type
Poster
Location
Upper Atrium/Hall
Presentation Date
15 Apr 2015, 1:00 pm - 3:00 pm
Analysis of Cellular Secretion via NMR and Toroid Cavity Probes
Upper Atrium/Hall
Yeast cells, such as Saccharomyces Cerevisiae, provide information for medical diagnostics, genetics, biochemical and microbiological applications. Due to structural, functional, and genetic similarities with higher level eukaryotic and human cells, yeast cells are common model cells. Yeast cells (3-4 μm in size) and their enzyme secretions should offer spectroscopic and spatial signatures via NMR and MRI. Initial experiment designs required the central conductor of a toroid cavity device (TCD) probe to be dip-coated in agar, exposed to yeast cells, and placed in incubation. After subsequent incubation periods, the central conductor was placed in a cylindrical TCD modified for insertion of the central conductor. Analysis utilized high field NMR spectrometers, and provided spectral information about the growth medium, secretions, and cells. The development of these new spectroscopic and imaging techniques for studies of cell secretion could be translated to the analysis of cancer cells.
