Title

Analysis of Cellular Secretion via NMR and Toroid Cavity Probes

Presenter Information

Sierra Herndon

Department

Chemistry

Major

Biological Sciences

Research Advisor

Woelk, Klaus
Gerald, Rex

Advisor's Department

Chemistry

Funding Source

Missouri S&T’s Opportunities for Undergraduate Research Experiences (OURE) Program

Abstract

Yeast cells, such as Saccharomyces Cerevisiae, provide information for medical diagnostics, genetics, biochemical and microbiological applications. Due to structural, functional, and genetic similarities with higher level eukaryotic and human cells, yeast cells are common model cells. Yeast cells (3-4 μm in size) and their enzyme secretions should offer spectroscopic and spatial signatures via NMR and MRI. Initial experiment designs required the central conductor of a toroid cavity device (TCD) probe to be dip-coated in agar, exposed to yeast cells, and placed in incubation. After subsequent incubation periods, the central conductor was placed in a cylindrical TCD modified for insertion of the central conductor. Analysis utilized high field NMR spectrometers, and provided spectral information about the growth medium, secretions, and cells. The development of these new spectroscopic and imaging techniques for studies of cell secretion could be translated to the analysis of cancer cells.

Biography

Sierra Herndon is an incoming Freshman studying Biological Sciences at the University of Missouri Science and Technology. She has been working with Dr. Woelk’s and Dr. Gerald’s research group since August 2014 with the Nuclear Magnetic Resonance spectrometers and electronics.

Research Category

Research Proposals

Presentation Type

Poster Presentation

Document Type

Poster

Location

Upper Atrium/Hall

Presentation Date

15 Apr 2015, 1:00 pm - 3:00 pm

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Apr 15th, 1:00 PM Apr 15th, 3:00 PM

Analysis of Cellular Secretion via NMR and Toroid Cavity Probes

Upper Atrium/Hall

Yeast cells, such as Saccharomyces Cerevisiae, provide information for medical diagnostics, genetics, biochemical and microbiological applications. Due to structural, functional, and genetic similarities with higher level eukaryotic and human cells, yeast cells are common model cells. Yeast cells (3-4 μm in size) and their enzyme secretions should offer spectroscopic and spatial signatures via NMR and MRI. Initial experiment designs required the central conductor of a toroid cavity device (TCD) probe to be dip-coated in agar, exposed to yeast cells, and placed in incubation. After subsequent incubation periods, the central conductor was placed in a cylindrical TCD modified for insertion of the central conductor. Analysis utilized high field NMR spectrometers, and provided spectral information about the growth medium, secretions, and cells. The development of these new spectroscopic and imaging techniques for studies of cell secretion could be translated to the analysis of cancer cells.