The Fibrillation Kinetics of Human and Bovine Insulin Fragments and Tailored Peptides

Amer Al-Lozi

Department

Chemical and Biochemical Engineering

Major

Chemical Engineering (Biology emphasis)

Research Advisor

Forciniti, Daniel

Advisor's Department

Chemical and Biochemical Engineering

Abstract

Amyloid deposits are insoluble proteinaceous fibrils that are responsible for diseases such as Alzheimer’s disease, type 2 diabetes and Parkinson’s disease. These proteins form clumps, due to misfolding, known as amyloid plaques that interfere with neuronal function. This study was focused on the monitoring of the kinetics of the insoluble proteins, starting from oligomers to mature fibrils. An experimental design was made to determine the main factors that contribute to fibrillation. A Thioflavin T fluorescence assay was performed to determine the presence of amyloid deposits. Afterwards, FT-IR (Fourier Transform Infrared Spectroscopy) was used to determine concentration of amyloid deposits.

Biography

Amer Al-Lozi is a Chemical Engineering student researching under Dr. Forciniti.

Research Category

Engineering

Presentation Type

Poster Presentation

Document Type

Poster

Location

Upper Atrium/Hall

Presentation Date

15 Apr 2015, 1:00 pm - 3:00 pm

Comments

Joint project with Amy Snyder

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Apr 15th, 1:00 PM Apr 15th, 3:00 PM

The Fibrillation Kinetics of Human and Bovine Insulin Fragments and Tailored Peptides

Upper Atrium/Hall

Amyloid deposits are insoluble proteinaceous fibrils that are responsible for diseases such as Alzheimer’s disease, type 2 diabetes and Parkinson’s disease. These proteins form clumps, due to misfolding, known as amyloid plaques that interfere with neuronal function. This study was focused on the monitoring of the kinetics of the insoluble proteins, starting from oligomers to mature fibrils. An experimental design was made to determine the main factors that contribute to fibrillation. A Thioflavin T fluorescence assay was performed to determine the presence of amyloid deposits. Afterwards, FT-IR (Fourier Transform Infrared Spectroscopy) was used to determine concentration of amyloid deposits.