Title

Constitutive Activity in Orphan G-Protein Coupled Receptors

Presenter Information

Michael A. Steurer

Department

Chemistry

Major

Chemistry, Biochemistry Emphasis Area

Research Advisor

Martin, Adam
Aronstam, Robert

Advisor's Department

Chemistry

Second Advisor's Department

Biological Sciences

Abstract

The G-protein coupled receptor type is the most diversely expressed in our genome, and their characterization is an important avenue of ongoing pharmacological research. GPCRs are most commonly identified by the endogenous ligand with which they interact. For many of those identified, however, an endogenous ligand is not known. Sequence relationship methods have identified families based on similarity in their ligand-binding site, but require more information before the endogenous ligand can be inferred. This research classifies several of these orphan GPCRs through the analysis of their constitutive activity on the adenylyl cyclase pathway. Signaling was monitored by transfecting cells with a reporter gene construct under control of the CRE promoter in conjunction with a particular orphan receptor vector plasmid. Results of this analysis reveal the potential role of the selected orphan receptors, and provides direction for further research in determining their endogenous ligand.

Biography

Michael A. Steurer, informally known as Abe, has been an involved member of the cDNA resource center of the Missouri S&T biology department for over two years. Guided by his interest in neuroscience, he has sought to apply his biochemical studies to the understanding of this system. In the long term, he hopes to pursue a career in pharmacological research. He was fortunate in that the opportunity for undergraduate research in which he has participated in aligns closely with his long term research goals.

Research Category

Sciences

Presentation Type

Oral Presentation

Document Type

Presentation

Location

Gasconade Room

Presentation Date

15 Apr 2015, 11:00 am - 11:30 am

This document is currently not available here.

Share

COinS
 
Apr 15th, 11:00 AM Apr 15th, 11:30 AM

Constitutive Activity in Orphan G-Protein Coupled Receptors

Gasconade Room

The G-protein coupled receptor type is the most diversely expressed in our genome, and their characterization is an important avenue of ongoing pharmacological research. GPCRs are most commonly identified by the endogenous ligand with which they interact. For many of those identified, however, an endogenous ligand is not known. Sequence relationship methods have identified families based on similarity in their ligand-binding site, but require more information before the endogenous ligand can be inferred. This research classifies several of these orphan GPCRs through the analysis of their constitutive activity on the adenylyl cyclase pathway. Signaling was monitored by transfecting cells with a reporter gene construct under control of the CRE promoter in conjunction with a particular orphan receptor vector plasmid. Results of this analysis reveal the potential role of the selected orphan receptors, and provides direction for further research in determining their endogenous ligand.