Title

Angiogenic Effects of Variant Lengths of Borate Glass Scaffolds Prepared via Robocasting

Presenter Information

Caleb Trecazzi

Department

Biological Sciences

Major

Chemistry (Emphasis in Pre-med) and Biology

Research Advisor

Day, D. E.
Rahaman, M. N., 1950-

Advisor's Department

Materials Science and Engineering

Funding Source

OURE Fellows

Abstract

Borate-based 13-93B3 bioactive glass scaffolds have been used successfully in clinical trials to treat chronic wounds and have been shown to be both safe and effective in promoting angiogenesis both in vivo and in vitro. The objective of this research was to determine the degree of capillary and blood vessel growth present within bioactive glass scaffolds between five and twenty millimeters in length when constructed via robocasting. The scaffolds were implanted subcutaneously in rats using aseptic surgery techniques. The reacted bioactive scaffolds were extracted and prepared for histological analysis through paraffin embedding and tissue sectioning followed by hematoxylin and eosin (H&E) and periodic acid-Schiff (PAS) staining for sample evaluation.

Biography

Caleb Trecazzi is a senior undergraduate student pursuing a dual bachelor’s degree in pre-medical chemistry and biology with a minor in applied mathematics. He is a member of Phi Sigma, the biology honor society, and Kappa Mu Epsilon, the mathematics honor society. Caleb has experience with molecular genetics techniques from his work in the iGEM laboratory and microbiology techniques from his brief work under graduate student Tiffany Edwards in the environmental microbiology laboratory. He has worked with Dr. Matt Insall in the mathematics department on lattice theory and nonstandard analysis during a previous summer research project in mathematics.

Presentation Type

OURE Fellows Final Oral Presentation

Document Type

Presentation

Location

Gasconade Room

Presentation Date

15 Apr 2015, 2:40 pm - 3:00 pm

Comments

Joint project with Anthony Bitar

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Apr 15th, 2:40 PM Apr 15th, 3:00 PM

Angiogenic Effects of Variant Lengths of Borate Glass Scaffolds Prepared via Robocasting

Gasconade Room

Borate-based 13-93B3 bioactive glass scaffolds have been used successfully in clinical trials to treat chronic wounds and have been shown to be both safe and effective in promoting angiogenesis both in vivo and in vitro. The objective of this research was to determine the degree of capillary and blood vessel growth present within bioactive glass scaffolds between five and twenty millimeters in length when constructed via robocasting. The scaffolds were implanted subcutaneously in rats using aseptic surgery techniques. The reacted bioactive scaffolds were extracted and prepared for histological analysis through paraffin embedding and tissue sectioning followed by hematoxylin and eosin (H&E) and periodic acid-Schiff (PAS) staining for sample evaluation.