Title

Angiogenic Effects of Variant Lengths of Borate Glass Scaffolds Prepared via Robocasting

Presenter Information

Anthony Bitar

Department

Biological Sciences

Major

Pre-medical Biology

Research Advisor

Day, D. E.
Rahaman, M. N., 1950-

Advisor's Department

Materials Science and Engineering

Funding Source

OURE Fellows

Abstract

Borate-based 13-93B3 bioactive glass scaffolds have been used successfully in clinical trials to treat chronic wounds and have been shown to be both safe and effective in promoting angiogenesis both in vivo and in vitro. The objective of this research was to determine the degree of capillary and blood vessel growth present within bioactive glass scaffolds between five and twenty millimeters in length when constructed via robocasting. The scaffolds were implanted subcutaneously in rats using aseptic surgery techniques. The reacted bioactive scaffolds were extracted and prepared for histological analysis through paraffin embedding and tissue sectioning followed by hematoxylin and eosin (H&E) and periodic acid-Schiff (PAS) staining for sample evaluation.

Biography

Anthony Bitar is a sophomore who is pursuing a Pre-Medical Bachelor’s degree in the Biological Sciences department, and minoring in Chemistry and Biomedical Engineering. He has worked under the supervision of Dr. Mohamed Rahaman and Dr. Yue-Wern Huang since February 2014. Anthony has worked in making Hydroxyapatite hollow microscpheres as well as bioactive glass scaffolds via robocasting. He has learned cell culturing techniques which enables him to conduct in vitro experiments. Additionally, Anthony has experience in conducting in vivo studies related to bone regeneration and angiogenesis using Sprague Dawley laboratory rats. He also gained the skills of performing histological analysis of implants.

Presentation Type

Poster Presentation

Document Type

Poster

Location

Upper Atrium/Hall

Presentation Date

15 Apr 2015, 9:00 am - 11:45 am

Comments

Joint project with Caleb Trecazzi

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Apr 15th, 9:00 AM Apr 15th, 11:45 AM

Angiogenic Effects of Variant Lengths of Borate Glass Scaffolds Prepared via Robocasting

Upper Atrium/Hall

Borate-based 13-93B3 bioactive glass scaffolds have been used successfully in clinical trials to treat chronic wounds and have been shown to be both safe and effective in promoting angiogenesis both in vivo and in vitro. The objective of this research was to determine the degree of capillary and blood vessel growth present within bioactive glass scaffolds between five and twenty millimeters in length when constructed via robocasting. The scaffolds were implanted subcutaneously in rats using aseptic surgery techniques. The reacted bioactive scaffolds were extracted and prepared for histological analysis through paraffin embedding and tissue sectioning followed by hematoxylin and eosin (H&E) and periodic acid-Schiff (PAS) staining for sample evaluation.