Title

Quorum Sensing inBradyrhizobium Japonicum

Presenter Information

Jeremiah Herbert

Department

Biological Sciences

Major

Biological Sciences

Research Advisor

Westenberg, David J.

Advisor's Department

Biological Sciences

Funding Source

Missouri S&T Opportunities for Undergraduate Research Experiences (OURE) Program

Abstract

Bradyrhizobium japonicum is a soil dwelling bacterium that is known to nodulate soybean roots and conduct nitrogen fixation for the plant. We hypothesize that for this to happen, a process known as quorum sensing is important. Quorum sensing is a density dependent process, and it is the way the way that B. japonicum communicate with each other. We predict that B. japonicum must have a gene that controls production of the quorum sensing molecule. By finding this gene, it may be possible to use this information to better use B. japonicum as a natural fertilizer. Companies currently sell pre-inoculated seedlings that grow and nodulate correctly in the lab, but in real world application the plants do not nodulate correctly. We believe that this may be because the plants are pre-inoculated at high concentrations, which allows quorum sensing molecules to be released at high concentrations. This high concentration of quorum sensing molecules may hinder the ability of the bacteria to nodulate properly.

Biography

Jeremiah is from Belleville, IL, and is a senior in Biological Sciences set to graduate in May of 2014. He is the Vice President of Fellowship for the Christian Campus Fellowship and is actively involved with Helix. He plans to return to school for his Master’s degree after working in the biotechnology/biology industry, and eventually getting his Ph.D before moving to biomedical research.

Research Category

Sciences

Presentation Type

Poster Presentation

Document Type

Poster

Location

Upper Atrium/Hall

Presentation Date

16 Apr 2014, 9:00 am - 11:45 am

Comments

Joint project with Adrian Black

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Apr 16th, 9:00 AM Apr 16th, 11:45 AM

Quorum Sensing inBradyrhizobium Japonicum

Upper Atrium/Hall

Bradyrhizobium japonicum is a soil dwelling bacterium that is known to nodulate soybean roots and conduct nitrogen fixation for the plant. We hypothesize that for this to happen, a process known as quorum sensing is important. Quorum sensing is a density dependent process, and it is the way the way that B. japonicum communicate with each other. We predict that B. japonicum must have a gene that controls production of the quorum sensing molecule. By finding this gene, it may be possible to use this information to better use B. japonicum as a natural fertilizer. Companies currently sell pre-inoculated seedlings that grow and nodulate correctly in the lab, but in real world application the plants do not nodulate correctly. We believe that this may be because the plants are pre-inoculated at high concentrations, which allows quorum sensing molecules to be released at high concentrations. This high concentration of quorum sensing molecules may hinder the ability of the bacteria to nodulate properly.