Title

Determining the Function of the RGCt Domain of IQG1 in Budding Yeast

Presenter Information

Kelsey Crossen

Department

Biological Sciences

Major

Biological Sciences

Research Advisor

Shannon, Katie

Advisor's Department

Biological Sciences

Funding Source

Opportunities for Undergraduate Experience Program (OURE)

Abstract

Cytokinesis is the final step in cell division, when the mother cell divides into two daughter cells. In budding yeast, IQG1 is an extracellular scaffolding protein that is required for formation and contraction of the actin ring during cytokinesis. The Ras GAP C-terminus domain (RGCt) is one of the four domains found in IQG1. This domain is highly conserved in IQGAP family members, but the function of this domain in budding yeast is currently unknown. Based on data from the human homolog, we suspected that this domain would be required for binding to actin-nucleating proteins called formins; however, our preliminary data shows that this is not the case. Deletion of the RGCt domain is lethal to yeast cells, so its function is essential. To determine binding partners for this domain, we will purify a His-tagged RGCt protein from bacteria, incubate with yeast extracts, and identify candidate interacting proteins by western blotting and novel interactions using mass spectrometry.

Biography

Kelsey Crossen is a junior majoring in Biological Sciences with minors in Chemistry and Psychology. She has been an undergraduate researcher in Dr. Katie Shannon’s Cytokinesis Lab since February 2013. Kelsey is the secretary and a lab instructor for S&T’s International Genetically Engineered Machine Team, a member of Phi Sigma Biological Sciences Honors Fraternity, and works for the Bio Sci department as a grader and as the BioConnection writer. After graduating from S&T next year, she plans to enter graduate school to study Microbiology or Cell Biology.

Research Category

Sciences

Presentation Type

Poster Presentation

Document Type

Poster

Location

Upper Atrium/Hall

Presentation Date

16 Apr 2014, 9:00 am - 11:45 am

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Apr 16th, 9:00 AM Apr 16th, 11:45 AM

Determining the Function of the RGCt Domain of IQG1 in Budding Yeast

Upper Atrium/Hall

Cytokinesis is the final step in cell division, when the mother cell divides into two daughter cells. In budding yeast, IQG1 is an extracellular scaffolding protein that is required for formation and contraction of the actin ring during cytokinesis. The Ras GAP C-terminus domain (RGCt) is one of the four domains found in IQG1. This domain is highly conserved in IQGAP family members, but the function of this domain in budding yeast is currently unknown. Based on data from the human homolog, we suspected that this domain would be required for binding to actin-nucleating proteins called formins; however, our preliminary data shows that this is not the case. Deletion of the RGCt domain is lethal to yeast cells, so its function is essential. To determine binding partners for this domain, we will purify a His-tagged RGCt protein from bacteria, incubate with yeast extracts, and identify candidate interacting proteins by western blotting and novel interactions using mass spectrometry.