Title

Fibril Formation by Human and Bovine Insulin Fragments

Presenter Information

Min kyung Cho

Department

Chemical and Biochemical Engineering

Major

Chemical Engineering

Research Advisor

Forciniti, Daniel

Advisor's Department

Chemical and Biochemical Engineering

Funding Source

Missouri S& T Opportunities for Undergraduate Research Experiences (OURE) Program

Abstract

Many diseases such as Alzheimer's and Parkinson's are linked to amyloid deposits, which are insoluble protein aggregates. It is known that their formation damages tissue, but the kinetics of their formation is not well understood. Factors affecting protein aggregation include concentration, agitation, and pH. Bovine and human insulin fragments known to form aggregates were synthesized. Solutions of the fragments were frozen and thaw to induce the formation of fibrils. In addition, experiments were designed to incubate solutions of the fragments in a water bath under constant aeration. Unfortunately, this set of experiments has not been completed yet. Congo and ThT staining were used to detect fibril formation but the results were inconclusive.

Biography

Min kyung is from South Korea, is a senior in Chemical Engineering at Missouri University of Science and Technology. She is the president of Korean Student Association for 2012-2013. She is graduating in May, 2013 and plans to study cosmetic engineering in graduate school.

Research Category

Engineering

Presentation Type

Poster Presentation

Document Type

Poster

Location

Upper Atrium/Hallway

Presentation Date

03 Apr 2013, 1:00 pm - 3:00 pm

This document is currently not available here.

Share

COinS
 
Apr 3rd, 1:00 PM Apr 3rd, 3:00 PM

Fibril Formation by Human and Bovine Insulin Fragments

Upper Atrium/Hallway

Many diseases such as Alzheimer's and Parkinson's are linked to amyloid deposits, which are insoluble protein aggregates. It is known that their formation damages tissue, but the kinetics of their formation is not well understood. Factors affecting protein aggregation include concentration, agitation, and pH. Bovine and human insulin fragments known to form aggregates were synthesized. Solutions of the fragments were frozen and thaw to induce the formation of fibrils. In addition, experiments were designed to incubate solutions of the fragments in a water bath under constant aeration. Unfortunately, this set of experiments has not been completed yet. Congo and ThT staining were used to detect fibril formation but the results were inconclusive.