Title

Investigating the Negative Regulation of Iqg1 in Budding Yeast Cytokinesis

Presenter Information

Avery Joseph

Department

Biological Sciences

Major

Biology

Research Advisor

Shannon, Katie

Advisor's Department

Biological Sciences

Funding Source

Grants acquired by Dr. Shannon

Abstract

Cytokinesis is the separation of cells and is the final step involved in division of the cell. In budding yeast (S. cerevisiae) the protein Iqg1 helps regulate the actin cytoskeleton involved in the contraction ring necessary for cytokinesis. Iqg1 is similar to the IQGAP1 found in humans, which can be more active in tumor cells. The experiment being conducted involves the negatively regulated interaction of Iqg1 with GTPase Tem1 necessary for contraction of the myosin ring. It is hypothesized that the proteins Bfa1 and Bub2 required for the spindle checkpoint regulates the Iqg1-Tem1 interaction. To test this, cells without Bfa1and Bub2 or overexpression of these proteins will be studied to establish what effects occur on the Iqg1-Tem1 co-immunoprecipitation.

Biography

Avery is a junior in the Biology department. She has been a member of SCRUBS, iGEM, and is currently the Awards Chair for Order of Omega and the Ritual Chair in Zeta Tau Alpha. Avery plans on pursuing a degree in Medicine post-graduation.

Research Category

Sciences

Presentation Type

Poster Presentation

Document Type

Poster

Location

Upper Atrium/Hallway

Presentation Date

10 Apr 2012, 9:00 am - 11:45 am

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Apr 10th, 9:00 AM Apr 10th, 11:45 AM

Investigating the Negative Regulation of Iqg1 in Budding Yeast Cytokinesis

Upper Atrium/Hallway

Cytokinesis is the separation of cells and is the final step involved in division of the cell. In budding yeast (S. cerevisiae) the protein Iqg1 helps regulate the actin cytoskeleton involved in the contraction ring necessary for cytokinesis. Iqg1 is similar to the IQGAP1 found in humans, which can be more active in tumor cells. The experiment being conducted involves the negatively regulated interaction of Iqg1 with GTPase Tem1 necessary for contraction of the myosin ring. It is hypothesized that the proteins Bfa1 and Bub2 required for the spindle checkpoint regulates the Iqg1-Tem1 interaction. To test this, cells without Bfa1and Bub2 or overexpression of these proteins will be studied to establish what effects occur on the Iqg1-Tem1 co-immunoprecipitation.