Title

The Therapeutic Effects of the Novel Thiol Antioxidant, N-acetylcysteine Amide (NACA), on Treating Rats with Grade I and Grade II Cataracts

Presenter Information

Tiffany Edwards

Department

Chemistry

Major

Biology

Research Advisor

Ercal, Nuran
Tobwala, Shakila

Advisor's Department

Chemistry

Funding Source

Ercal Vitek Endowment and NIH

Abstract

The effect of the antioxidant N-acetylcysteine amide (NACA) on the reversal of grades I and II cataracts in Wistar rat pups was evaluated. Cataract formation was induced via a glutathione inhibitor, l-buthionine-(S,R)-sulfoximine (BSO). The lens of each pup was tested for a number of oxidative stress related parameters, including the observation of glutathione and cysteine levels. After the pups opened their eyes, the grades of cataracts were evaluated in each lens. These pups were then divided into three groups; BSO only, grade I cataracts and grade II cataracts. The two groups that were treated with NACA eye drops showed a significant reduction of the cataracts. Our findings indicate that NACA is able to reverse BSO-induced cataracts (Grades I&II) by acting as a substrate donor for the synthesis of glutathione. In addition, NACA can also function as a scavenger for dangerous free radicals in the cells.

Biography

Tiffany is a junior majoring in Biology. She has been working in the laboratory of Dr. Ercal for two years. She plans on attending graduate school in Stockholm, Sweden. She has an avid interest in working biology and chemistry.

Research Category

Sciences

Presentation Type

Poster Presentation

Document Type

Poster

Award

Sciences poster session, First place

Location

Upper Atrium/Hallway

Presentation Date

10 Apr 2012, 9:00 am - 11:45 am

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Apr 10th, 9:00 AM Apr 10th, 11:45 AM

The Therapeutic Effects of the Novel Thiol Antioxidant, N-acetylcysteine Amide (NACA), on Treating Rats with Grade I and Grade II Cataracts

Upper Atrium/Hallway

The effect of the antioxidant N-acetylcysteine amide (NACA) on the reversal of grades I and II cataracts in Wistar rat pups was evaluated. Cataract formation was induced via a glutathione inhibitor, l-buthionine-(S,R)-sulfoximine (BSO). The lens of each pup was tested for a number of oxidative stress related parameters, including the observation of glutathione and cysteine levels. After the pups opened their eyes, the grades of cataracts were evaluated in each lens. These pups were then divided into three groups; BSO only, grade I cataracts and grade II cataracts. The two groups that were treated with NACA eye drops showed a significant reduction of the cataracts. Our findings indicate that NACA is able to reverse BSO-induced cataracts (Grades I&II) by acting as a substrate donor for the synthesis of glutathione. In addition, NACA can also function as a scavenger for dangerous free radicals in the cells.