Title

Use of Endophytic Bacteria for Growth Promotion and Toxicity Resistance in Leachate Treated Poplar Trees

Presenter Information

Ashley Muehler

Department

Biological Sciences

Major

Biological Sciences

Research Advisor

Westenberg, David J.

Advisor's Department

Biological Sciences

Funding Source

Missouri S&T Opportunities for Undergraduate Research Experiences (OURE) Program; NIH - NIEHS Superfund Research Program, Project # 5R01ES016158

Abstract

The goal of this research is to determine if Enterobacter 638, Mycobacterium vanbaalenii PYR-1, or Methylobacterium populi can improve growth and toxicity resistance in poplar trees used for phytoremediation of landfill leachate. Phytoremediation of the leachate can help prevent contamination of surrounding soil and water. Cuttings of three different poplar tree hybrids were inoculated with the endophytes, planted and watered with various concentrations of leachate. The growth and water usage of the trees were recorded and trees were harvested at different time intervals. Root samples were obtained and placed in a freezer to save for analysis. The roots were surface sterilized and processed for plating and incubation. We hypothesized that, tolerance to the leachate would correlate with increased endosymbiont growth and predicted that recovery of the inoculants from the root samples should also increase.

Biography

Ashley Muehler is a junior at Missouri University of Science and Technology. She is pursuing her Bachelors of Science degree in Biological Sciences with a minor in Chemistry. Ashley is a member of the national honor society, Phi Kappa Phi. She is also a member of the biological sciences honor society of Phi Sigma and life sciences club Helix. Ashley is pursuing a career in the research industry.

Research Category

Sciences

Presentation Type

Poster Presentation

Document Type

Poster

Award

Sciences poster session, First place

Location

Upper Atrium/Hallway

Presentation Date

07 Apr 2010, 9:00 am - 11:45 am

Comments

Joint project with Crystal Halloran

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Apr 7th, 9:00 AM Apr 7th, 11:45 AM

Use of Endophytic Bacteria for Growth Promotion and Toxicity Resistance in Leachate Treated Poplar Trees

Upper Atrium/Hallway

The goal of this research is to determine if Enterobacter 638, Mycobacterium vanbaalenii PYR-1, or Methylobacterium populi can improve growth and toxicity resistance in poplar trees used for phytoremediation of landfill leachate. Phytoremediation of the leachate can help prevent contamination of surrounding soil and water. Cuttings of three different poplar tree hybrids were inoculated with the endophytes, planted and watered with various concentrations of leachate. The growth and water usage of the trees were recorded and trees were harvested at different time intervals. Root samples were obtained and placed in a freezer to save for analysis. The roots were surface sterilized and processed for plating and incubation. We hypothesized that, tolerance to the leachate would correlate with increased endosymbiont growth and predicted that recovery of the inoculants from the root samples should also increase.