Conversion of Borate-Based Glass Scaffold to Hydroxyapatite in a Dilute Phosphate Solution


Porous scaffolds of a borate-based glass (composition in mol%: 6Na2O, 8K2O, 8MgO, 22CaO, 36B2O3, 18SiO2, 2P2O5), with interconnected porosity of ~70% and pores of size 200-500 µm, were prepared by a polymer foam replication technique. The degradation of the scaffolds and conversion to a hydroxyapatite-type material in a 0.02 M K2HPO4 solution (starting pH = 7.0) at 37 °C were studied by measuring the weight loss of the scaffolds, as well as the pH and the boron concentration of the solution. X-ray diffraction, scanning electronic microscopy and energy dispersive x-ray analysis showed that a hydroxyapatite-type material was formed on the glass surface within 7 days of immersion in the phosphate solution. Cellular response to the scaffolds was assessed using murine MLO-A5 cells, an osteogenic cell line. Scanning electron microscopy showed that the scaffolds supported cell attachment and proliferation during the 6 day incubation. The results indicate that this borate-based glass could provide a promising degradable scaffold material for bone tissue engineering applications.


Nuclear Engineering and Radiation Science

Second Department

Materials Science and Engineering

Keywords and Phrases

Animals; Bone Substitutes/Chemical Synthesis; Borates/Chemistry; Cell Line; Cell Proliferation; Cell Survival; Crystallization/Methods; Durapatite/Chemistry; Glass/Chemistry; Materials Testing; Mice; Osteoblasts/Cytology; Osteoblasts/ Physiology; Phosphates/Chemistry; Solutions; Surface Properties; Tissue Engineering/Methods; Tissue Scaffolds

International Standard Serial Number (ISSN)


Document Type

Article - Journal

Document Version


File Type





© 2010 Institute of Physics - IOP Publishing, All rights reserved.

Publication Date

01 Jan 2010