Enhanced Bone Regeneration in Rat Calvarial Defects Implanted with Surface-Modified and BMP-Loaded Bioactive Glass (13-93) Scaffolds
The repair of large bone defects, such as segmental defects in the long bones of the limbs, is a challenging clinical problem. Our recent work has shown the ability to create porous scaffolds of silicate 13-93 bioactive glass by robocasting which have compressive strengths comparable to human cortical bone. The objective of this study was to evaluate the capacity of those strong porous scaffolds with a grid-like microstructure (porosity = 50%; filament width = 330 μm; pore width = 300 μm) to regenerate bone in a rat calvarial defect model. Six weeks post-implantation, the amount of new bone formed within the implants was evaluated using histomorphometric analysis. The amount of new bone formed in implants composed of the as-fabricated scaffolds was 32% of the available pore space (area). Pretreating the as-fabricated scaffolds in an aqueous phosphate solution for 1, 3 and 6 days to convert a surface layer to hydroxyapatite prior to implantation enhanced new bone formation to 46%, 57% and 45%, respectively. New bone formation in scaffolds pretreated for 1, 3 and 6 days and loaded with bone morphogenetic protein-2 (BMP-2) (1 μg per defect) was 65%, 61% and 64%, respectively. The results show that converting a surface layer of the glass to hydroxyapatite or loading the surface-treated scaffolds with BMP-2 can significantly improve the capacity of 13-93 bioactive glass scaffolds to regenerate bone in an osseous defect. Based on their mechanical properties evaluated previously and their capacity to regenerate bone found in this study, these 13-93 bioactive glass scaffolds, pretreated or loaded with BMP-2, are promising in structural bone repair.
X. Liu et al., "Enhanced Bone Regeneration in Rat Calvarial Defects Implanted with Surface-Modified and BMP-Loaded Bioactive Glass (13-93) Scaffolds," Acta Biomaterialia, vol. 9, no. 7, pp. 7506-7517, Elsevier, Jan 2013.
The definitive version is available at https://doi.org/10.1016/j.actbio.2013.03.039
Nuclear Engineering and Radiation Science
Materials Science and Engineering
Keywords and Phrases
Bone Regeneration; Bioactive Glass Scaffold; Surface Modification; Bone Morphogenetic Protein-2; Rat Calvarial Defect Model
International Standard Serial Number (ISSN)
Article - Journal
© 2013 Elsevier, All rights reserved.
01 Jan 2013