Column Liquid Chromatography
The objective of the current study was the development and subsequent validation of a simple, sensitive, precise and stability-indicating reversed-phase HPLC method for the determination of ciprofloxacin HCl in pharmaceutical dosage forms in the presence of its potential impurities. The chromatographic separation of ciprofloxacin HCl and its related compounds was achieved on an Inertsil ODS3 column using UV detection. The optimized mobile phase consisted of phosphoric acid solution: acetonitril. The proposed method provided linear responses within the concentration range 250-750 µg mL ˉ¹ for ciprofloxacin HCl and 0.5-1.5 µg mLˉ¹ for its related compounds. LOD and LOQ values for the active substance were 5.159 and 15.632 µg mLˉ¹, respectively. Correlation coefficients (r) of the regression equations for the impurities were greater than 0.99 in all cases. The precision of the method was demonstrated using intra- and inter-day assay RSD% values which were less than 1% in all instances. No interference from any components of pharmaceutical dosage forms or degradation products was observed. 2007 Friedr. Vieweg & Sohn Verlag/GWV Fachverlage GmbH.
B. S. Aksoy et al., "Column Liquid Chromatography," Chromatographia, Springer Verlag, Jan 2007.
The definitive version is available at https://doi.org/10.1365/s10337-007-0287-6
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© 2007 Springer Verlag, All rights reserved.
01 Jan 2007