Bioactive Borate Glass Scaffold for Bone Tissue Engineering


Borate glass particles and microspheres with size distributions in the range of approximately 100-400 μm, were loosely compacted and sintered for 10 min at 600 °C to form a porous, three-dimensional construct (porosity 25-40%). Conversion of the borate glass to hydroxyapatite was investigated by soaking the constructs in a solution of K2HPO4 (0.25 M) at 37 °C and with a pH value of 9.0, and measuring the weight loss of the constructs as a function of time. Almost full conversion of the borate glass to hydroxyapatite was achieved in less than 6 days. X-ray diffraction revealed an initially amorphous product that subsequently crystallized to hydroxyapatite. The biocompatibility of the porous constructs was investigated by in vitro cell culture with human mesenchymal stem cells derived from bone marrow (bMSC) and human mesenchymal stem cell derived osteoblasts (MSC-Ob). The cells adhered to the scaffolds and the MSC-Obs produced alkaline phosphatase which is an indication of osteogenic differentiation. The data suggest strong bioactive characteristics for the borate glass constructs and the potential use of the constructs as scaffolds for tissue engineering of bone.


Materials Science and Engineering

Keywords and Phrases

Bioglass; Borates; Porosity; Scanning electron microscopy

International Standard Serial Number (ISSN)

0022-3093; 1873-4812

Document Type

Article - Journal

Document Version


File Type





© 2008 Elsevier, All rights reserved.

Publication Date

01 Mar 2008